Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 954164..954818 Replicon chromosome
Accession NZ_CP091704
Organism Escherichia coli strain KGu016

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag L6L59_RS04635 Protein ID WP_000244781.1
Coordinates 954411..954818 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L6L59_RS04630 Protein ID WP_000354046.1
Coordinates 954164..954430 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L59_RS04610 (950252) 950252..951685 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
L6L59_RS04615 (951730) 951730..952041 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
L6L59_RS04620 (952205) 952205..952864 + 660 WP_000250274.1 hemolysin III family protein -
L6L59_RS04625 (952941) 952941..953921 - 981 WP_000886079.1 tRNA-modifying protein YgfZ -
L6L59_RS04630 (954164) 954164..954430 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L6L59_RS04635 (954411) 954411..954818 + 408 WP_000244781.1 protein YgfX Toxin
L6L59_RS04640 (954858) 954858..955379 - 522 WP_001055874.1 flavodoxin FldB -
L6L59_RS04645 (955491) 955491..956387 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
L6L59_RS04650 (956412) 956412..957122 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L6L59_RS04655 (957128) 957128..958861 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T234621 WP_000244781.1 NZ_CP091704:954411-954818 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T234621 NZ_LR133917:c839460-839272 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT234621 WP_000354046.1 NZ_CP091704:954164-954430 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT234621 NZ_LR133917:c839749-839489 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References