Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3902206..3902860 Replicon chromosome
Accession NZ_CP091700
Organism Escherichia coli strain KFu019

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag L6L55_RS20140 Protein ID WP_000244777.1
Coordinates 3902206..3902613 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L6L55_RS20145 Protein ID WP_000354046.1
Coordinates 3902594..3902860 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L55_RS20120 (3898163) 3898163..3899896 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
L6L55_RS20125 (3899902) 3899902..3900612 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L6L55_RS20130 (3900637) 3900637..3901533 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
L6L55_RS20135 (3901645) 3901645..3902166 + 522 WP_001055874.1 flavodoxin FldB -
L6L55_RS20140 (3902206) 3902206..3902613 - 408 WP_000244777.1 protein YgfX Toxin
L6L55_RS20145 (3902594) 3902594..3902860 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L6L55_RS20150 (3903103) 3903103..3904083 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
L6L55_RS20155 (3904279) 3904279..3904938 - 660 WP_000250274.1 hemolysin III family protein -
L6L55_RS20160 (3905102) 3905102..3905413 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
L6L55_RS20165 (3905458) 3905458..3906891 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
L6L55_RS20170 (3906948) 3906948..3907691 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T234587 WP_000244777.1 NZ_CP091700:c3902613-3902206 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T234587 NZ_LR130562:c4433506-4433216 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT234587 WP_000354046.1 NZ_CP091700:c3902860-3902594 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT234587 NZ_LR130562:c4433738-4433496 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References