Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3579837..3580491 Replicon chromosome
Accession NZ_CP091677
Organism Escherichia coli strain KFu022

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag L6L66_RS17515 Protein ID WP_000244777.1
Coordinates 3579837..3580244 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L6L66_RS17520 Protein ID WP_000354046.1
Coordinates 3580225..3580491 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L66_RS17495 (3575794) 3575794..3577527 - 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -
L6L66_RS17500 (3577533) 3577533..3578243 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L6L66_RS17505 (3578268) 3578268..3579164 - 897 WP_000806628.1 site-specific tyrosine recombinase XerD -
L6L66_RS17510 (3579276) 3579276..3579797 + 522 WP_001055874.1 flavodoxin FldB -
L6L66_RS17515 (3579837) 3579837..3580244 - 408 WP_000244777.1 protein YgfX Toxin
L6L66_RS17520 (3580225) 3580225..3580491 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L6L66_RS17525 (3580734) 3580734..3581714 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
L6L66_RS17530 (3581910) 3581910..3582569 - 660 WP_000250274.1 hemolysin III family protein -
L6L66_RS17535 (3582733) 3582733..3583044 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
L6L66_RS17540 (3583089) 3583089..3584522 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
L6L66_RS17545 (3584579) 3584579..3585322 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T234478 WP_000244777.1 NZ_CP091677:c3580244-3579837 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T234478 NZ_LR130545:c2994713-2994462 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT234478 WP_000354046.1 NZ_CP091677:c3580491-3580225 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT234478 NZ_LR130545:c2994465-2994211 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References