Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 3240..3762 Replicon plasmid pKKo009_3
Accession NZ_CP091674
Organism Escherichia coli strain KKo009

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag L6L68_RS24585 Protein ID WP_000220561.1
Coordinates 3240..3521 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag L6L68_RS24590 Protein ID WP_000121743.1
Coordinates 3511..3762 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L68_RS24570 (L6L68_24565) 1346..1534 + 189 WP_000957857.1 hypothetical protein -
L6L68_RS24575 (L6L68_24570) 1544..2743 + 1200 WP_000948429.1 IS91 family transposase -
L6L68_RS24580 (L6L68_24575) 2909..3001 + 93 Protein_3 IS6 family transposase -
L6L68_RS24585 (L6L68_24580) 3240..3521 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L6L68_RS24590 (L6L68_24585) 3511..3762 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
L6L68_RS24595 (L6L68_24590) 4997..5836 + 840 WP_024129959.1 replication initiation protein -
L6L68_RS24600 (L6L68_24595) 5857..6087 + 231 WP_063090927.1 hypothetical protein -
L6L68_RS24610 (L6L68_24605) 6895..7476 + 582 WP_235638730.1 replication initiation protein -
L6L68_RS24615 (L6L68_24610) 7480..7926 + 447 WP_001074384.1 hypothetical protein -
L6L68_RS24620 (L6L68_24615) 8063..8416 + 354 WP_024129958.1 DNA distortion polypeptide 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-IIa - 1..18520 18520
- inside IScluster/Tn - - 1544..6795 5251


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T234453 WP_000220561.1 NZ_CP091674:c3521-3240 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T234453 NZ_LR130543:c5291329-5290946 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT234453 WP_000121743.1 NZ_CP091674:c3762-3511 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT234453 NZ_LR130543:c5291571-5291329 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References