Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3236462..3237087 Replicon chromosome
Accession NZ_CP091673
Organism Escherichia coli strain KKo009

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag L6L68_RS16820 Protein ID WP_000911329.1
Coordinates 3236462..3236860 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag L6L68_RS16825 Protein ID WP_000450524.1
Coordinates 3236860..3237087 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L68_RS16800 (3232369) 3232369..3232569 + 201 WP_000383836.1 YpfN family protein -
L6L68_RS16805 (3232650) 3232650..3233348 - 699 WP_000679819.1 esterase -
L6L68_RS16810 (3233422) 3233422..3235437 - 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
L6L68_RS16815 (3235452) 3235452..3236315 - 864 WP_001267498.1 neutral zinc metallopeptidase -
L6L68_RS16820 (3236462) 3236462..3236860 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
L6L68_RS16825 (3236860) 3236860..3237087 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
L6L68_RS16830 (3237244) 3237244..3237957 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
L6L68_RS16835 (3238170) 3238170..3239204 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
L6L68_RS16840 (3239221) 3239221..3240099 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
L6L68_RS16845 (3240245) 3240245..3240817 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
L6L68_RS16850 (3240817) 3240817..3241287 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T234440 WP_000911329.1 NZ_CP091673:c3236860-3236462 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T234440 NZ_LR130541:c5377551-5377168 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT234440 WP_000450524.1 NZ_CP091673:c3237087-3236860 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT234440 NZ_LR130541:c5377793-5377551 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References