Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3243143..3243761 Replicon chromosome
Accession NZ_CP091659
Organism Escherichia coli strain KFu024

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag L6L61_RS16615 Protein ID WP_001291435.1
Coordinates 3243143..3243361 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag L6L61_RS16620 Protein ID WP_000344800.1
Coordinates 3243387..3243761 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L61_RS16580 (3238432) 3238432..3239004 + 573 WP_000779826.1 YbaY family lipoprotein -
L6L61_RS16585 (3239035) 3239035..3239346 - 312 WP_000409911.1 MGMT family protein -
L6L61_RS16595 (3239725) 3239725..3240078 + 354 WP_000878141.1 DUF1428 family protein -
L6L61_RS16600 (3240120) 3240120..3241670 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
L6L61_RS16605 (3241834) 3241834..3242304 - 471 WP_000136192.1 YlaC family protein -
L6L61_RS16610 (3242420) 3242420..3242971 - 552 WP_000102564.1 maltose O-acetyltransferase -
L6L61_RS16615 (3243143) 3243143..3243361 - 219 WP_001291435.1 HHA domain-containing protein Toxin
L6L61_RS16620 (3243387) 3243387..3243761 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
L6L61_RS16625 (3244307) 3244307..3247456 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
L6L61_RS16630 (3247479) 3247479..3248672 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T234317 WP_001291435.1 NZ_CP091659:c3243361-3243143 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T234317 NZ_LR130515:c2687910-2687644 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT234317 WP_000344800.1 NZ_CP091659:c3243761-3243387 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT234317 NZ_LR130515:c2688161-2687910 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References