Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 1676100..1676900 Replicon chromosome
Accession NZ_CP091659
Organism Escherichia coli strain KFu024

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag L6L61_RS09095 Protein ID WP_000342449.1
Coordinates 1676100..1676627 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag L6L61_RS09100 Protein ID WP_001277108.1
Coordinates 1676634..1676900 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L61_RS09070 (1671175) 1671175..1671942 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
L6L61_RS09075 (1671939) 1671939..1673216 - 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
L6L61_RS09080 (1673213) 1673213..1674139 - 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
L6L61_RS09085 (1674187) 1674187..1675296 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
L6L61_RS09090 (1675720) 1675720..1676103 + 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
L6L61_RS09095 (1676100) 1676100..1676627 - 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
L6L61_RS09100 (1676634) 1676634..1676900 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
L6L61_RS09105 (1677050) 1677050..1678153 - 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
L6L61_RS09110 (1678424) 1678424..1679278 - 855 WP_000130221.1 RNA polymerase sigma factor RpoH -
L6L61_RS09115 (1679523) 1679523..1680581 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
L6L61_RS09120 (1680574) 1680574..1681242 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T234308 WP_000342449.1 NZ_CP091659:c1676627-1676100 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T234308 NZ_LR130515:c943747-943559 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT234308 WP_001277108.1 NZ_CP091659:c1676900-1676634 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT234308 NZ_LR130515:c944036-943776 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References