Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1376912..1377711 Replicon chromosome
Accession NZ_CP091659
Organism Escherichia coli strain KFu024

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag L6L61_RS07550 Protein ID WP_000347273.1
Coordinates 1377247..1377711 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L6L61_RS07545 Protein ID WP_001307405.1
Coordinates 1376912..1377247 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L61_RS07530 (1372697) 1372697..1373467 - 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -
L6L61_RS07535 (1373483) 1373483..1374817 - 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
L6L61_RS07540 (1375192) 1375192..1376763 + 1572 WP_001723936.1 galactarate dehydratase -
L6L61_RS07545 (1376912) 1376912..1377247 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L6L61_RS07550 (1377247) 1377247..1377711 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L6L61_RS07555 (1377766) 1377766..1378575 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L6L61_RS07560 (1378824) 1378824..1380104 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
L6L61_RS07565 (1380127) 1380127..1380600 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
L6L61_RS07570 (1380611) 1380611..1381390 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
L6L61_RS07575 (1381380) 1381380..1382258 + 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
L6L61_RS07580 (1382276) 1382276..1382710 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T234307 WP_000347273.1 NZ_CP091659:1377247-1377711 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T234307 NZ_LR130513:c2536864-2536598 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT234307 WP_001307405.1 NZ_CP091659:1376912-1377247 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT234307 NZ_LR130513:c2537121-2536864 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References