Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1829222..1829784 Replicon chromosome
Accession NC_011750
Organism Escherichia coli IAI39

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag ECIAI39_RS08780 Protein ID WP_000605676.1
Coordinates 1829506..1829784 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag ECIAI39_RS08775 Protein ID WP_000781370.1
Coordinates 1829222..1829506 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECIAI39_RS08760 1824581..1827628 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
ECIAI39_RS08765 1827641..1828525 + 885 WP_001240581.1 formate dehydrogenase N subunit beta -
ECIAI39_RS08770 1828518..1829171 + 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
ECIAI39_RS08775 1829222..1829506 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
ECIAI39_RS08780 1829506..1829784 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECIAI39_RS08785 1829970..1830980 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
ECIAI39_RS08790 1831114..1832811 - 1698 WP_000433462.1 malate dehydrogenase -
ECIAI39_RS08795 1832968..1833105 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
ECIAI39_RS08800 1833207..1833422 - 216 WP_000495767.1 biofilm-dependent modulation protein -
ECIAI39_RS08805 1833767..1834198 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T23430 WP_000605676.1 NC_011750:c1829784-1829506 [Escherichia coli IAI39]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T23430 NC_011750:c1829784-1829506 [Escherichia coli IAI39]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGATTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTCGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT23430 WP_000781370.1 NC_011750:c1829506-1829222 [Escherichia coli IAI39]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT23430 NC_011750:c1829506-1829222 [Escherichia coli IAI39]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCTCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References