Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2620271..2620896 Replicon chromosome
Accession NC_011741
Organism Escherichia coli IAI1

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag ECIAI1_RS12735 Protein ID WP_000911329.1
Coordinates 2620271..2620669 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag ECIAI1_RS12740 Protein ID WP_000450524.1
Coordinates 2620669..2620896 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECIAI1_RS12715 2616149..2616349 + 201 WP_000383836.1 YpfN family protein -
ECIAI1_RS12720 2616459..2617157 - 699 WP_000679823.1 esterase -
ECIAI1_RS12725 2617231..2619246 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
ECIAI1_RS12730 2619261..2620124 - 864 WP_001267498.1 neutral zinc metallopeptidase -
ECIAI1_RS12735 2620271..2620669 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
ECIAI1_RS12740 2620669..2620896 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
ECIAI1_RS12745 2621051..2621764 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
ECIAI1_RS12755 2621977..2623011 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
ECIAI1_RS12760 2623028..2623906 - 879 WP_001311023.1 4-hydroxy-tetrahydrodipicolinate synthase -
ECIAI1_RS12765 2624052..2624624 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
ECIAI1_RS12770 2624624..2625094 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T23340 WP_000911329.1 NC_011741:c2620669-2620271 [Escherichia coli IAI1]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T23340 NC_011741:c2620669-2620271 [Escherichia coli IAI1]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT23340 WP_000450524.1 NC_011741:c2620896-2620669 [Escherichia coli IAI1]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT23340 NC_011741:c2620896-2620669 [Escherichia coli IAI1]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References