Detailed information of TA system TA233118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4320813..4321434
• Replicon: chromosome
• Accession: NZ_CP091311
• Organism: Pseudomonas juntendi strain 18091276

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: L2Y89_RS20425
• Protein ID: WP_019819701.1
• Coordinates: 4320813..4321118 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: L2Y89_RS20430
• Protein ID: WP_001172026.1
• Coordinates: 4321099..4321434 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Y89_RS20410 (L2Y89_20410)	4316327..4316881	-	555	WP_003159191.1	aminoglycoside N-acetyltransferase AAC(6')-Ib4	-
L2Y89_RS20415 (L2Y89_20415)	4317029..4317769	-	741	WP_003159548.1	subclass B1 metallo-beta-lactamase IMP-1	-
L2Y89_RS20420 (L2Y89_20420)	4317922..4318935	+	1014	WP_000845048.1	class 1 integron integrase IntI1	-
L2Y89_RS20425 (L2Y89_20425)	4320813..4321118	+	306	WP_019819701.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L2Y89_RS20430 (L2Y89_20430)	4321099..4321434	+	336	WP_001172026.1	helix-turn-helix transcriptional regulator	Antitoxin
L2Y89_RS20435 (L2Y89_20435)	4321449..4321784	+	336	WP_000741275.1	hypothetical protein	-
L2Y89_RS20440 (L2Y89_20440)	4321808..4322134	+	327	WP_000091614.1	hypothetical protein	-
L2Y89_RS20445 (L2Y89_20445)	4322131..4322511	+	381	WP_001054412.1	hypothetical protein	-
L2Y89_RS20450 (L2Y89_20450)	4322668..4323024	+	357	WP_237144814.1	DUF3391 domain-containing protein	-
L2Y89_RS20455 (L2Y89_20455)	4323108..4323977	+	870	WP_237144306.1	HD-GYP domain-containing protein	-
L2Y89_RS20460 (L2Y89_20460)	4324307..4324726	+	420	WP_023662957.1	TIGR03757 family integrating conjugative element protein	-
L2Y89_RS20465 (L2Y89_20465)	4324726..4325661	+	936	WP_023662956.1	TIGR03756 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaIMP-1	-	4245783..4334416	88633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (1-85)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 10981.64 Da
• Isoelectric Point: 9.8622

>T233118 WP_019819701.1 NZ_CP091311:4320813-4321118 [Pseudomonas juntendi]
VRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAVFVLHCFQKKSKSGIATPKADMDII
RARLKVAEVLAQELRNAKTNH

Nucleotide

• Length: 306 bp

>T233118 NZ_CP128531:c2081900-2081511 [Mannheimia haemolytica]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGATTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGCGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT233118 WP_001172026.1 NZ_CP091311:4321099-4321434 [Pseudomonas juntendi]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT233118 NZ_CP128531:c2082161-2081901 [Mannheimia haemolytica]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAACAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAGAGAGAAACCAATCCTTTGAGCAACGG
GATCCTTTTGAGGTAGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7