Detailed information of TA system TA233115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 2737435..2738068
• Replicon: chromosome
• Accession: NZ_CP091308
• Organism: Burkholderia pyrrocinia strain LWK2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L2Y90_RS29895
• Protein ID: WP_258283750.1
• Coordinates: 2737655..2738068 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L2Y90_RS29890
• Protein ID: WP_258283749.1
• Coordinates: 2737435..2737668 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Y90_RS29880 (L2Y90_29825)	2734106..2736406	+	2301	WP_258283748.1	ATP-dependent DNA helicase	-
L2Y90_RS29885 (L2Y90_29830)	2736503..2737273	-	771	WP_072436791.1	IclR family transcriptional regulator C-terminal domain-containing protein	-
L2Y90_RS29890 (L2Y90_29835)	2737435..2737668	+	234	WP_258283749.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L2Y90_RS29895 (L2Y90_29840)	2737655..2738068	+	414	WP_258283750.1	PIN domain-containing protein	Toxin
L2Y90_RS29900 (L2Y90_29845)	2738128..2738739	+	612	WP_258283751.1	hypothetical protein	-
L2Y90_RS29905 (L2Y90_29850)	2738868..2740169	-	1302	WP_152857285.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
L2Y90_RS29910 (L2Y90_29855)	2740374..2741684	-	1311	WP_152857286.1	shikimate transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-120)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15146.32 Da
• Isoelectric Point: 6.0807

>T233115 WP_258283750.1 NZ_CP091308:2737655-2738068 [Burkholderia pyrrocinia]
MSGIESAKSFVDSNVVLYLLSGDETKANRAEALFLRRPTVSVQVLNEVASVCSRKLRMPWSDVGQFLEPIRSLCHVVPVT
IDTHDLARRLAERHGLSFHDACIVAAASLEGCEYLYTEDMRDGLRVDGGPVLRNPFA

Nucleotide

• Length: 414 bp

>T233115 NZ_CP128531:641177-641473 [Mannheimia haemolytica]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8863.08 Da
• Isoelectric Point: 8.8894

>AT233115 WP_258283749.1 NZ_CP091308:2737435-2737668 [Burkholderia pyrrocinia]
MQVAKWGNSLAVRLPASVVDALELREGDDIEIVVDSPRVFAVSRKPRPDELLQRLRRFRGRLPADFKFSRDDANERD

Nucleotide

• Length: 234 bp

>AT233115 NZ_CP128531:641457-641699 [Mannheimia haemolytica]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA