Detailed information of TA system TA233107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 102652..104482
• Replicon: chromosome
• Accession: NZ_CP091308
• Organism: Burkholderia pyrrocinia strain LWK2

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: L2Y90_RS17785
• Protein ID: WP_258284017.1
• Coordinates: 103586..104482 (-)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: L2Y90_RS17780
• Protein ID: WP_258284016.1
• Coordinates: 102652..103506 (-)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L2Y90_RS17760 (L2Y90_17720)	97731..99221	-	1491	WP_258284012.1	aldehyde dehydrogenase	-
L2Y90_RS17765 (L2Y90_17725)	99310..100125	-	816	WP_258284013.1	aspartate dehydrogenase	-
L2Y90_RS17770 (L2Y90_17730)	100118..101785	-	1668	WP_258284014.1	thiamine pyrophosphate-binding protein	-
L2Y90_RS17775 (L2Y90_17735)	101778..102575	-	798	WP_258284015.1	SDR family oxidoreductase	-
L2Y90_RS17780 (L2Y90_17740)	102652..103506	-	855	WP_258284016.1	IclR family transcriptional regulator	Antitoxin
L2Y90_RS17785 (L2Y90_17745)	103586..104482	-	897	WP_258284017.1	alpha/beta fold hydrolase	Toxin
L2Y90_RS17790 (L2Y90_17750)	104501..105028	-	528	WP_174421109.1	cupin domain-containing protein	-
L2Y90_RS17795 (L2Y90_17755)	105058..105828	-	771	WP_258284018.1	SDR family oxidoreductase	-
L2Y90_RS17800 (L2Y90_17760)	105821..106891	-	1071	WP_258284019.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
L2Y90_RS17805 (L2Y90_17765)	106947..107258	-	312	WP_152861025.1	hypothetical protein	-
L2Y90_RS17810 (L2Y90_17770)	107260..107577	-	318	WP_258284020.1	non-heme iron oxygenase ferredoxin subunit	-
L2Y90_RS17815 (L2Y90_17775)	108025..109104	+	1080	WP_258284021.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (51-279)

Antitoxin

• Transcription regulator IclR, C-terminal (142-265)

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 30767.10 Da
• Isoelectric Point: 7.7326

>T233107 WP_258284017.1 NZ_CP091308:c104482-103586 [Burkholderia pyrrocinia]
MSVIDKRERDRTAAFAALLGQFPEQRCAAGAVGTIGYREAGVQHAGRTLPVVLLHGIGSGAASWVRQLDMLGASRRVLAW
DAPGYGASTPVHGASPAAADYAASLNAWLEALGIERCVLVGHSLGAIVAGGLARVMPARIAGLLLVSPAGGYGSAPAETR
ESRRDARLAMLAELGPAGLAEQRSGNMLSGFASEDARAWVRWNMARIVPAGYAQATHLLANADLATDLAGFRGRTAVAVG
ANDAITPPAACERIAAAAHVGLQVIPQAGHAGYVEAPAVYSALIDTFCRQCDTQWGLV

Nucleotide

• Length: 897 bp

>T233107 NZ_CP128530:395780-396049 [Mannheimia haemolytica]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTTTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GCTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31334.86 Da
• Isoelectric Point: 7.3578

>AT233107 WP_258284016.1 NZ_CP091308:c103506-102652 [Burkholderia pyrrocinia]
MSDPLQQEPENGKAEASYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYKL
GIAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTTHIVIRDGRDVVFVAKAQSQSPVFSSIRVNVGTRLPAYATTHGHV
LMGDLSLKELRSLYPEGTLNRMTSATPETVDALYQVIREDARRGYGVSNSSFERGISVVTAPVRNETQKIVACITVTVPR
PEIDAALIADGLIDKVQRAAAELSRRLNYRSDDEHTFMKALGLK

Nucleotide

• Length: 855 bp

>AT233107 NZ_CP128530:395527-395793 [Mannheimia haemolytica]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA