Detailed information of TA system TA232690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1989..2463
• Replicon: plasmid pWSCRKP-5
• Accession: NZ_CP091073
• Organism: Klebsiella pneumoniae strain WSCRKP

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: L0980_RS29440
• Protein ID: WP_004197762.1
• Coordinates: 2197..2463 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: L0980_RS29435
• Protein ID: WP_000879771.1
• Coordinates: 1989..2213 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0980_RS29420 (L0980_29420)	98..343	-	246	WP_032433971.1	hypothetical protein	-
L0980_RS29425 (L0980_29425)	594..1103	+	510	WP_032433972.1	MbeB family mobilization protein	-
L0980_RS29430 (L0980_29430)	1110..1322	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
L0980_RS29435 (L0980_29435)	1989..2213	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
L0980_RS29440 (L0980_29440)	2197..2463	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0980_RS29445 (L0980_29445)	2533..2883	-	351	WP_004197756.1	hypothetical protein	-
L0980_RS29450 (L0980_29450)	2949..3494	-	546	WP_023317533.1	hypothetical protein	-
L0980_RS29455 (L0980_29455)	3520..3846	-	327	WP_050516389.1	hypothetical protein	-
L0980_RS29460 (L0980_29460)	5002..5178	+	177	WP_004197757.1	hypothetical protein	-
L0980_RS29465 (L0980_29465)	5168..5512	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T232690 WP_004197762.1 NZ_CP091073:2197-2463 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T232690 NZ_CP127881:c4124298-4123876 [Serratia marcescens]
GTGGCCCAGTGGCGATGTGATATCCGCATTTCCTGGCGCACCCAGCTGTTTTCACTGTTGACCCACGGTGTTCTGATCCT
GCTGATCCTGATTTCGCCCTGGCCGGAAGGCTTTGGCCCGCTCTGGCTGGTGCTGCTGACGCTGGTGGTGTTTCAGTGCA
TTCGCAGCCAGAAGCGCATCGCCGCGGTGCAGGGCGAGCTGCGGCTGCTGGCGGATCGGCGTTTCAGCTGGCACGGCCGC
GAGTGGCGGCTGGCGAAAAAACCGTGGATGCCGGGTTACGGCATGCTGTTGACGCTGCAGCCGATGGAAGGCAAAAAGCG
CCGCCGGCTGTGGCTGGTTTCCGACTGCATGAGCAAAGAGGAATGGCGCCACCTGCGGCAGCTGTTGCTGTATCCGCCGG
CGGGTGACGGCGAACAAGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT232690 WP_000879771.1 NZ_CP091073:1989-2213 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT232690 NZ_CP127881:c4124545-4124279 [Serratia marcescens]
ATGGATATTAACAACAAAGCACGTATTCACTGGGCTTGCCGCCGTGGCATGCGCGAGCTGGACATCTCCATCATGCCGTT
CTTCGAATACGAATACGACAGCCTGAATGACGCGGACAAGGCGCTGTTTATCCGCCTGCTGGAGTGCGACGATCCCGATT
TGTTCAACTGGCTGATGAATCATGGCGCGCCGCAGGACGGCGAGCTGCAGAGAATGGTGACCCTGATCCAAACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5