Detailed information of TA system TA232504

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 7385..7654
• Replicon: plasmid p2_STEC384
• Accession: NZ_CP091031
• Organism: Escherichia coli strain STEC384

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: L1P14_RS25705
• Protein ID: WP_001323520.1
• Coordinates: 7538..7654 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	7385..7450 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1P14_RS25665	2533..2679	+	147	Protein_4	adenine-specific DNA-methyltransferase	-
L1P14_RS25670	2730..3956	-	1227	WP_001484257.1	RNA-guided endonuclease TnpB family protein	-
L1P14_RS25675	4015..4419	+	405	WP_001175008.1	IS200/IS605 family transposase	-
L1P14_RS25680	4497..6011	+	1515	Protein_7	ParB/RepB/Spo0J family partition protein	-
L1P14_RS25685	6066..6500	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
L1P14_RS25690	6497..7259	+	763	Protein_9	plasmid SOS inhibition protein A	-
L1P14_RS25695	7228..7416	-	189	WP_032084246.1	hypothetical protein	-
-	7228..7452	+	225	NuclAT_0	-	-
-	7228..7452	+	225	NuclAT_0	-	-
-	7228..7452	+	225	NuclAT_0	-	-
-	7228..7452	+	225	NuclAT_0	-	-
-	7385..7450	-	66	-	-	Antitoxin
L1P14_RS25700	7438..7587	+	150	Protein_11	plasmid maintenance protein Mok	-
L1P14_RS25705	7538..7654	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
L1P14_RS25710	8071..8244	-	174	Protein_13	hypothetical protein	-
L1P14_RS25715	8314..8520	+	207	WP_000547968.1	hypothetical protein	-
L1P14_RS25720	8545..8831	+	287	Protein_15	hypothetical protein	-
L1P14_RS25725	8950..9771	+	822	WP_074014913.1	DUF932 domain-containing protein	-
L1P14_RS25730	10068..10670	-	603	WP_235418686.1	transglycosylase SLT domain-containing protein	-
L1P14_RS25735	10992..11375	+	384	WP_040073112.1	conjugal transfer relaxosome DNA-binding protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa	1..71807	71807
	-	flank	IS/Tn	-	-	2730..3962	1232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T232504 WP_001323520.1 NZ_CP091031:7538-7654 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T232504 NZ_CP127314:c1189444-1189067 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT232504 NZ_CP091031:c7450-7385 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin