Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 323755..324555 Replicon chromosome
Accession NZ_CP091024
Organism Escherichia coli strain STEC379

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag L1P13_RS01500 Protein ID WP_000342449.1
Coordinates 324028..324555 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag L1P13_RS01495 Protein ID WP_001277108.1
Coordinates 323755..324021 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1P13_RS01475 (319412) 319412..320080 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
L1P13_RS01480 (320073) 320073..321131 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
L1P13_RS01485 (321376) 321376..322230 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
L1P13_RS01490 (322501) 322501..323605 + 1105 Protein_295 branched chain amino acid ABC transporter substrate-binding protein LivJ -
L1P13_RS01495 (323755) 323755..324021 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
L1P13_RS01500 (324028) 324028..324555 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
L1P13_RS01505 (324552) 324552..324935 - 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
L1P13_RS01510 (325359) 325359..326468 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
L1P13_RS01515 (326516) 326516..327442 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
L1P13_RS01520 (327439) 327439..328716 + 1278 WP_000803784.1 branched chain amino acid ABC transporter permease LivM -
L1P13_RS01525 (328713) 328713..329480 + 768 WP_000082103.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T232416 WP_000342449.1 NZ_CP091024:324028-324555 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T232416 NZ_CP127277:3159693-3159956 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT232416 WP_001277108.1 NZ_CP091024:323755-324021 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT232416 NZ_CP127277:3159408-3159689 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References