Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4544970..4545565 Replicon chromosome
Accession NZ_CP091018
Organism Escherichia coli strain STEC307

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag L1O94_RS22945 Protein ID WP_000239579.1
Coordinates 4544970..4545320 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag L1O94_RS22950 Protein ID WP_001223208.1
Coordinates 4545314..4545565 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L1O94_RS22925 (4540246) 4540246..4541268 - 1023 WP_001339490.1 ABC transporter permease -
L1O94_RS22930 (4541282) 4541282..4542784 - 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
L1O94_RS22935 (4543094) 4543094..4544050 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
L1O94_RS22940 (4544360) 4544360..4544890 + 531 WP_000055072.1 inorganic diphosphatase -
L1O94_RS22945 (4544970) 4544970..4545320 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
L1O94_RS22950 (4545314) 4545314..4545565 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
L1O94_RS22955 (4545778) 4545778..4546119 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
L1O94_RS22960 (4546122) 4546122..4549901 - 3780 WP_000060921.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T232354 WP_000239579.1 NZ_CP091018:c4545320-4544970 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T232354 NZ_CP127276:c3095331-3094975 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT232354 WP_001223208.1 NZ_CP091018:c4545565-4545314 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT232354 NZ_CP127276:c3095545-3095315 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References