Detailed information of TA system TA232348

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2204497..2204868
• Replicon: chromosome
• Accession: NZ_CP091018
• Organism: Escherichia coli strain STEC307

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: L1O94_RS10875
• Protein ID: WP_021500490.1
• Coordinates: 2204674..2204868 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2204497..2204675 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L1O94_RS10835 (2199939)	2199939..2200595	-	657	WP_000011658.1	carbon-nitrogen hydrolase family protein YobB	-
L1O94_RS10840 (2200697)	2200697..2200927	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
L1O94_RS10845 (2201066)	2201066..2201440	+	375	WP_000204699.1	CopC domain-containing protein YobA	-
L1O94_RS10850 (2201444)	2201444..2202316	+	873	WP_033813010.1	copper homeostasis membrane protein CopD	-
L1O94_RS10855 (2202329)	2202329..2202670	+	342	WP_000976492.1	YebY family protein	-
L1O94_RS10860 (2203063)	2203063..2204139	-	1077	WP_063103093.1	phage integrase Arm DNA-binding domain-containing protein	-
L1O94_RS10865 (2204105)	2204105..2204386	-	282	WP_137689817.1	excisionase	-
- (2204497)	2204497..2204675	+	179	NuclAT_0	-	Antitoxin
- (2204497)	2204497..2204675	+	179	NuclAT_0	-	Antitoxin
- (2204497)	2204497..2204675	+	179	NuclAT_0	-	Antitoxin
- (2204497)	2204497..2204675	+	179	NuclAT_0	-	Antitoxin
L1O94_RS10870 (2204493)	2204493..2204681	-	189	WP_001356607.1	DUF1187 family protein	-
L1O94_RS10875 (2204674)	2204674..2204868	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
L1O94_RS10880 (2204932)	2204932..2205984	-	1053	WP_001004422.1	RecT family recombinase	-
L1O94_RS10885 (2205996)	2205996..2209118	-	3123	WP_063119603.1	exodeoxyribonuclease VIII	-
L1O94_RS10890 (2209220)	2209220..2209495	-	276	WP_000632298.1	hypothetical protein	-
L1O94_RS10895 (2209570)	2209570..2209740	-	171	WP_077633604.1	YdaE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2197196..2258070	60874

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T232348 WP_021500490.1 NZ_CP091018:c2204868-2204674 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T232348 NZ_CP127276:2853668-2854045 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT232348 NZ_CP091018:2204497-2204675 [Escherichia coli]
GATGACAGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin