Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2855476..2856101 Replicon chromosome
Accession NC_011415
Organism Escherichia coli SE11

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag ECSE_RS14355 Protein ID WP_000911329.1
Coordinates 2855476..2855874 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag ECSE_RS14360 Protein ID WP_000450524.1
Coordinates 2855874..2856101 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSE_RS14335 2851354..2851554 + 201 WP_000383836.1 YpfN family protein -
ECSE_RS14340 2851664..2852362 - 699 WP_000679823.1 esterase -
ECSE_RS14345 2852436..2854451 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
ECSE_RS14350 2854466..2855329 - 864 WP_001267498.1 neutral zinc metallopeptidase -
ECSE_RS14355 2855476..2855874 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
ECSE_RS14360 2855874..2856101 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
ECSE_RS14365 2856256..2856969 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
ECSE_RS14375 2857182..2858216 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
ECSE_RS14380 2858233..2859111 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
ECSE_RS14385 2859257..2859829 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
ECSE_RS14390 2859829..2860299 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T23216 WP_000911329.1 NC_011415:c2855874-2855476 [Escherichia coli SE11]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T23216 NC_011415:c2855874-2855476 [Escherichia coli SE11]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT23216 WP_000450524.1 NC_011415:c2856101-2855874 [Escherichia coli SE11]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT23216 NC_011415:c2856101-2855874 [Escherichia coli SE11]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References