Detailed information of TA system TA023193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1353738..1354329
• Replicon: chromosome
• Accession: NC_011386
• Organism: Oligotropha carboxidovorans OM5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B6JBF6
• Locus tag: OCAR_RS06405
• Protein ID: WP_012562526.1
• Coordinates: 1354048..1354329 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OCAR_RS06400
• Protein ID: WP_012562525.1
• Coordinates: 1353738..1354037 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCAR_RS06380	1349119..1351092	-	1974	WP_012562520.1	hypothetical protein	-
OCAR_RS06385	1351143..1352201	-	1059	WP_012562521.1	flippase-like domain-containing protein	-
OCAR_RS06390	1352607..1353113	+	507	WP_013913263.1	ferritin-like domain-containing protein	-
OCAR_RS06395	1353153..1353596	-	444	WP_041559641.1	hypothetical protein	-
OCAR_RS06400	1353738..1354037	-	300	WP_012562525.1	HigA family addiction module antidote protein	Antitoxin
OCAR_RS06405	1354048..1354329	-	282	WP_012562526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCAR_RS06410	1354419..1354949	-	531	WP_012562527.1	histidine phosphatase family protein	-
OCAR_RS06415	1355065..1356468	+	1404	WP_012562528.1	FAD-binding oxidoreductase	-
OCAR_RS06420	1356561..1357055	+	495	WP_012562529.1	peptide-methionine (R)-S-oxide reductase MsrB	-
OCAR_RS06425	1357235..1359043	+	1809	WP_012562530.1	flagellar hook-basal body complex protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10569.07 Da
• Isoelectric Point: 10.2043

>T23193 WP_012562526.1 NC_011386:c1354329-1354048 [Afipia carboxidovorans OM5]
VIKSFKDDLTEAAFNGKARKGFPAQLLKVARRKLAMVNAATSLDDLRSPPNNRLEALTRERAGQHSIRVNDQFRICFIWT
KDGAEQVELVDYH

Nucleotide

• Length: 282 bp

>T23193 NC_011386:c1354329-1354048 [Afipia carboxidovorans OM5]
GTGATCAAGAGCTTCAAGGACGATCTGACTGAAGCAGCCTTCAACGGGAAGGCACGCAAAGGTTTTCCGGCTCAGTTACT
CAAAGTTGCCCGCCGCAAACTGGCGATGGTGAATGCGGCTACATCCCTGGATGATCTGCGTTCGCCGCCCAACAACCGAC
TTGAAGCTCTCACCAGGGAGCGAGCGGGCCAGCATTCAATTCGGGTGAACGACCAGTTTCGCATCTGCTTCATATGGACG
AAGGATGGAGCTGAACAAGTCGAGCTTGTCGATTATCATTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10856.59 Da
• Isoelectric Point: 9.0146

>AT23193 WP_012562525.1 NC_011386:c1354037-1353738 [Afipia carboxidovorans OM5]
MAKTLPPLHPGEVLREEYLEPLNLSPGKLAKACGIPRTRIERIASERFGITADTALRLGKALGTSAQFWLNLQNDYDLRV
AKVAIAKDLEKVTSVQEAA

Nucleotide

• Length: 300 bp

>AT23193 NC_011386:c1354037-1353738 [Afipia carboxidovorans OM5]
ATGGCCAAGACACTGCCGCCACTGCACCCCGGGGAAGTTCTTCGGGAAGAATATCTGGAGCCGCTGAATCTCTCGCCCGG
CAAACTTGCCAAGGCCTGTGGCATTCCCCGTACGCGCATCGAGCGTATCGCCAGCGAGCGTTTCGGCATTACCGCAGACA
CTGCTCTACGACTCGGCAAGGCGCTCGGTACTTCCGCCCAGTTCTGGCTCAACCTGCAGAATGACTACGATCTGCGGGTG
GCAAAAGTGGCAATCGCGAAGGATCTGGAAAAGGTCACCTCCGTTCAAGAGGCCGCCTAG

Structures

Toxin

• AlphaFold DB: B6JBF6