Detailed information of TA system TA231796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 775309..775886
• Replicon: chromosome
• Accession: NZ_CP090748
• Organism: Burkholderia multivorans strain 2010Ycf503

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z02_RS19800
• Protein ID: WP_053298683.1
• Coordinates: 775309..775674 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S9ML45
• Locus tag: L0Z02_RS19805
• Protein ID: WP_035956044.1
• Coordinates: 775668..775886 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z02_RS19770 (L0Z02_19770)	770485..771384	+	900	WP_035487807.1	lipid A hydroxylase LpxO	-
L0Z02_RS19775 (L0Z02_19775)	771508..772236	+	729	WP_170933583.1	hypothetical protein	-
L0Z02_RS19780 (L0Z02_19780)	772298..773896	-	1599	WP_059936581.1	alkyl hydroperoxide reductase subunit F	-
L0Z02_RS19785 (L0Z02_19785)	774015..774578	-	564	WP_006399806.1	alkyl hydroperoxide reductase subunit C	-
L0Z02_RS19790 (L0Z02_19790)	774828..774986	+	159	WP_200053153.1	hypothetical protein	-
L0Z02_RS19795 (L0Z02_19795)	775025..775213	-	189	WP_200053152.1	hypothetical protein	-
L0Z02_RS19800 (L0Z02_19800)	775309..775674	-	366	WP_053298683.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z02_RS19805 (L0Z02_19805)	775668..775886	-	219	WP_035956044.1	ribbon-helix-helix protein, CopG family	Antitoxin
L0Z02_RS19810 (L0Z02_19810)	775988..776884	-	897	WP_217123441.1	LysR substrate-binding domain-containing protein	-
L0Z02_RS19815 (L0Z02_19815)	777060..778226	+	1167	WP_006403832.1	branched-chain amino acid ABC transporter substrate-binding protein	-
L0Z02_RS19820 (L0Z02_19820)	778395..778934	+	540	WP_105838720.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
L0Z02_RS19825 (L0Z02_19825)	778927..780198	+	1272	WP_249468552.1	Zn-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13556.55 Da
• Isoelectric Point: 5.1515

>T231796 WP_053298683.1 NZ_CP090748:c775674-775309 [Burkholderia multivorans]
MVKALFDTNILIDYLSGVESARLELGRYDHRAISTITWMEVLVGTTADDEGPIRAWLSSFDVIPLDSTVADRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPSTEPGIRVPYQI

Nucleotide

• Length: 366 bp

>T231796 NZ_CP126952:3010934-3011428 [Escherichia coli O78:H4]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGATCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8231.38 Da
• Isoelectric Point: 4.7640

>AT231796 WP_035956044.1 NZ_CP090748:c775886-775668 [Burkholderia multivorans]
MSRVLVDLSDGQLDELAVIVETEHRPRAAIIRDAIDAYIALHKRPLADDVFGLWKDRAVDGLAYQEKLRSEW

Nucleotide

• Length: 219 bp

>AT231796 NZ_CP126952:3010644-3010937 [Escherichia coli O78:H4]
ATGCCAGCAGCCAATAACATTTCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTATCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2S9ML45