Detailed information of TA system TA231674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 1934230..1934850
• Replicon: chromosome
• Accession: NZ_CP090702
• Organism: Burkholderia multivorans strain 2016Y70910410I

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: L0Z36_RS26770
• Protein ID: WP_249471220.1
• Coordinates: 1934230..1934547 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: B9B269
• Locus tag: L0Z36_RS26775
• Protein ID: WP_006398012.1
• Coordinates: 1934551..1934850 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z36_RS26760 (L0Z36_26760)	1930763..1932196	-	1434	WP_006404214.1	aldehyde dehydrogenase family protein	-
L0Z36_RS26765 (L0Z36_26765)	1932225..1933865	-	1641	WP_249471217.1	acetolactate synthase large subunit	-
L0Z36_RS26770 (L0Z36_26770)	1934230..1934547	+	318	WP_249471220.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Z36_RS26775 (L0Z36_26775)	1934551..1934850	+	300	WP_006398012.1	putative addiction module antidote protein	Antitoxin
L0Z36_RS26780 (L0Z36_26780)	1934885..1936171	-	1287	WP_053297867.1	DUF445 domain-containing protein	-
L0Z36_RS26785 (L0Z36_26785)	1936286..1937506	-	1221	WP_217091696.1	anthranilate 1,2-dioxygenase system ferredoxin--NAD(+) reductase	-
L0Z36_RS26790 (L0Z36_26790)	1937496..1937822	-	327	WP_249471223.1	anthranilate 1,2-dioxygenase ferredoxin subunit AndAb	-
L0Z36_RS26795 (L0Z36_26795)	1937840..1938328	-	489	WP_006398008.1	anthranilate 1,2-dioxygenase small subunit AndAd	-
L0Z36_RS26800 (L0Z36_26800)	1938343..1939614	-	1272	WP_249471230.1	anthranilate 1,2-dioxygenase large subunit AndAc	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-89)

Antitoxin

• Uncharacterized protein HI1420 (4-96)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12140.97 Da
• Isoelectric Point: 10.2349

>T231674 WP_249471220.1 NZ_CP090702:1934230-1934547 [Burkholderia multivorans]
MPYLAPTFSIRTTAVFDAWFASLQDRTAKRRIQARIDRLSMGNLGNWKSVGSPVYEMRIDHGPGYRVYFVRRRTIWVVLL
CGGDKSTQRDDIRAAHAMLEHLDLE

Nucleotide

• Length: 318 bp

>T231674 NZ_CP126942:2089787-2090038 [Escherichia coli O1:K1:H7]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10605.00 Da
• Isoelectric Point: 6.6381

>AT231674 WP_006398012.1 NZ_CP090702:1934551-1934850 [Burkholderia multivorans]
MSKISTRPWDSAEHLKTDDDMAEYFEACLQEGGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT231674 NZ_CP126942:2090035-2090289 [Escherichia coli O1:K1:H7]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3KTI5