Detailed information of TA system TA231652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1465925..1466502
• Replicon: chromosome
• Accession: NZ_CP090696
• Organism: Burkholderia multivorans strain 2017Y71078597II

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z09_RS22290
• Protein ID: WP_249461355.1
• Coordinates: 1466137..1466502 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S9ML45
• Locus tag: L0Z09_RS22285
• Protein ID: WP_035956044.1
• Coordinates: 1465925..1466143 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z09_RS22265 (L0Z09_22265)	1461564..1462835	-	1272	WP_249461346.1	Zn-dependent hydrolase	-
L0Z09_RS22270 (L0Z09_22270)	1462828..1463367	-	540	WP_249461349.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
L0Z09_RS22275 (L0Z09_22275)	1463585..1464751	-	1167	WP_249461352.1	branched-chain amino acid ABC transporter substrate-binding protein	-
L0Z09_RS22280 (L0Z09_22280)	1464927..1465823	+	897	WP_105794177.1	LysR substrate-binding domain-containing protein	-
L0Z09_RS22285 (L0Z09_22285)	1465925..1466143	+	219	WP_035956044.1	ribbon-helix-helix protein, CopG family	Antitoxin
L0Z09_RS22290 (L0Z09_22290)	1466137..1466502	+	366	WP_249461355.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z09_RS22295 (L0Z09_22295)	1466598..1466786	+	189	WP_200053152.1	hypothetical protein	-
L0Z09_RS22300 (L0Z09_22300)	1466825..1466983	-	159	WP_200053153.1	hypothetical protein	-
L0Z09_RS22305 (L0Z09_22305)	1467233..1467796	+	564	WP_006399806.1	alkyl hydroperoxide reductase subunit C	-
L0Z09_RS22310 (L0Z09_22310)	1467915..1469513	+	1599	WP_249461358.1	alkyl hydroperoxide reductase subunit F	-
L0Z09_RS22315 (L0Z09_22315)	1469575..1470315	-	741	WP_249461361.1	hypothetical protein	-
L0Z09_RS22320 (L0Z09_22320)	1470439..1471338	-	900	WP_249461365.1	lipid A hydroxylase LpxO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13625.66 Da
• Isoelectric Point: 5.8683

>T231652 WP_249461355.1 NZ_CP090696:1466137-1466502 [Burkholderia multivorans]
MVKALFDTNILIDYLSGVESARLELGRYDHRAISTITWMEVLVGTTADDEGPIRAWLSSFDVIPLDITVADRAVTIRKER
RIRLPDAIVWASAQVNGRLLVSRNTKDFPSTEPGIRVPYQI

Nucleotide

• Length: 366 bp

>T231652 NZ_CP126940:2629557-2630879 [Escherichia coli O2:K1:H7]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGAGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAATGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCAAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8231.38 Da
• Isoelectric Point: 4.7640

>AT231652 WP_035956044.1 NZ_CP090696:1465925-1466143 [Burkholderia multivorans]
MSRVLVDLSDGQLDELAVIVETEHRPRAAIIRDAIDAYIALHKRPLADDVFGLWKDRAVDGLAYQEKLRSEW

Nucleotide

• Length: 219 bp

>AT231652 NZ_CP126940:2629291-2629557 [Escherichia coli O2:K1:H7]
ATGATGAGCTTTCAGAAGATCTATAGCCCAATGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTAACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCAAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2S9ML45