Detailed information of TA system TA231650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 725676..726331
• Replicon: chromosome
• Accession: NZ_CP090696
• Organism: Burkholderia multivorans strain 2017Y71078597II

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L0Z09_RS19040
• Protein ID: WP_006409935.1
• Coordinates: 725912..726331 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L0Z09_RS19035
• Protein ID: WP_249462451.1
• Coordinates: 725676..725915 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z09_RS19010 (L0Z09_19010)	720737..720880	-	144	WP_006409932.1	hypothetical protein	-
L0Z09_RS19015 (L0Z09_19015)	721469..721672	+	204	WP_006403067.1	hypothetical protein	-
L0Z09_RS19020 (L0Z09_19020)	721699..722370	-	672	Protein_602	Fe2+-dependent dioxygenase	-
L0Z09_RS19025 (L0Z09_19025)	722367..723122	-	756	WP_006403069.1	tetratricopeptide repeat protein	-
L0Z09_RS19030 (L0Z09_19030)	723126..725372	-	2247	WP_006403070.1	TonB-dependent siderophore receptor	-
L0Z09_RS19035 (L0Z09_19035)	725676..725915	+	240	WP_249462451.1	Arc family DNA-binding protein	Antitoxin
L0Z09_RS19040 (L0Z09_19040)	725912..726331	+	420	WP_006409935.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z09_RS19045 (L0Z09_19045)	726465..727760	-	1296	WP_249462452.1	aspartate carbamoyltransferase	-
L0Z09_RS19050 (L0Z09_19050)	727993..728127	+	135	WP_006409923.1	entericidin A/B family lipoprotein	-
L0Z09_RS19055 (L0Z09_19055)	728221..728796	-	576	WP_053298362.1	hypothetical protein	-
L0Z09_RS19060 (L0Z09_19060)	728992..729942	-	951	WP_217057526.1	DMT family transporter	-
L0Z09_RS19065 (L0Z09_19065)	730188..730982	-	795	WP_105777245.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15146.38 Da
• Isoelectric Point: 5.2157

>T231650 WP_006409935.1 NZ_CP090696:725912-726331 [Burkholderia multivorans]
MILVDTNVIFEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLEQSIEHRVVPVFRGRILPFDD
AAGKAYARIRAHARAVGNAIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPWAS

Nucleotide

• Length: 420 bp

>T231650 NZ_CP126940:c2076333-2075959 [Escherichia coli O2:K1:H7]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8788.06 Da
• Isoelectric Point: 5.7066

>AT231650 WP_249462451.1 NZ_CP090696:725676-725915 [Burkholderia multivorans]
MPVITVRNLPDEVHRALRVRAALHGRSIEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDLDIQRDKTRTDPMSFE

Nucleotide

• Length: 240 bp

>AT231650 NZ_CP126940:c2076790-2076422 [Escherichia coli O2:K1:H7]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA