Detailed information of TA system TA231648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 3287774..3288411
• Replicon: chromosome
• Accession: NZ_CP090695
• Organism: Burkholderia multivorans strain 2017Y71078597II

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8E2UU98
• Locus tag: L0Z09_RS15640
• Protein ID: WP_035489546.1
• Coordinates: 3287774..3288175 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A132EWB1
• Locus tag: L0Z09_RS15645
• Protein ID: WP_006408845.1
• Coordinates: 3288175..3288411 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Z09_RS15630 (L0Z09_15630)	3285095..3286237	+	1143	WP_053296686.1	alginate lyase family protein	-
L0Z09_RS15635 (L0Z09_15635)	3286264..3287652	+	1389	WP_006408285.1	L-serine ammonia-lyase	-
L0Z09_RS15640 (L0Z09_15640)	3287774..3288175	-	402	WP_035489546.1	type II toxin-antitoxin system VapC family toxin	Toxin
L0Z09_RS15645 (L0Z09_15645)	3288175..3288411	-	237	WP_006408845.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L0Z09_RS15650 (L0Z09_15650)	3288599..3290302	+	1704	WP_006408280.1	thiamine pyrophosphate-binding protein	-
L0Z09_RS15655 (L0Z09_15655)	3290299..3291396	+	1098	WP_105761444.1	ADP-heptose--LPS heptosyltransferase	-
L0Z09_RS15660 (L0Z09_15660)	3291515..3292546	-	1032	WP_249463905.1	helix-turn-helix domain-containing protein	-
L0Z09_RS15665 (L0Z09_15665)	3292717..3293259	-	543	WP_006408276.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15035.28 Da
• Isoelectric Point: 5.7429

>T231648 WP_035489546.1 NZ_CP090695:c3288175-3287774 [Burkholderia multivorans]
MPRYMLDTNMCIYLMKNQPEQVARRFAQCYTGDVVMSAITYAELEYGVTLCTNPARERRNLAALIEDIPVAPFDVAAAQA
YGPIREATRERKKDHLDKLIGAHAVSLDVVLVTNNERDFASYPGLRLENWLIG

Nucleotide

• Length: 402 bp

>T231648 NZ_CP126940:2065636-2065887 [Escherichia coli O2:K1:H7]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8915.00 Da
• Isoelectric Point: 5.8651

>AT231648 WP_006408845.1 NZ_CP090695:c3288411-3288175 [Burkholderia multivorans]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERVGDEIRIRPARRPLTGVLGKFAKFGPDFMAEGRGDQEQAEREGW

Nucleotide

• Length: 237 bp

>AT231648 NZ_CP126940:2065884-2066138 [Escherichia coli O2:K1:H7]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A132EWB1