Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4076442..4077169 Replicon chromosome
Accession NC_011353
Organism Escherichia coli O157:H7 str. EC4115

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag ECH74115_RS21920 Protein ID WP_000550189.1
Coordinates 4076855..4077169 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ECH74115_RS21915 Protein ID WP_000560249.1
Coordinates 4076442..4076858 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECH74115_RS21905 4071502..4073853 + 2352 WP_000695517.1 alpha-glucosidase -
ECH74115_RS21910 4074379..4076397 + 2019 WP_000121479.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
ECH74115_RS21915 4076442..4076858 - 417 WP_000560249.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
ECH74115_RS21920 4076855..4077169 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
ECH74115_RS21930 4077453..4078589 - 1137 WP_000018678.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
ECH74115_RS21935 4078674..4079177 + 504 WP_001295542.1 M48 family metallopeptidase -
ECH74115_RS21940 4079254..4079946 + 693 WP_000942543.1 vancomycin high temperature exclusion protein -
ECH74115_RS21945 4080025..4081011 + 987 WP_000617675.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T23161 WP_000550189.1 NC_011353:c4077169-4076855 [Escherichia coli O157:H7 str. EC4115]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T23161 NC_011353:c4077169-4076855 [Escherichia coli O157:H7 str. EC4115]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15016.48 Da        Isoelectric Point: 4.5805

>AT23161 WP_000560249.1 NC_011353:c4076858-4076442 [Escherichia coli O157:H7 str. EC4115]
MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT23161 NC_011353:c4076858-4076442 [Escherichia coli O157:H7 str. EC4115]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References