Detailed information of TA system TA231590

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1073875..1074456
• Replicon: chromosome
• Accession: NZ_CP090673
• Organism: Burkholderia multivorans strain 2020Y71628512IV

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: L0Y82_RS15200
• Protein ID: WP_035946056.1
• Coordinates: 1074163..1074456 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J4SGI3
• Locus tag: L0Y82_RS15195
• Protein ID: WP_006409266.1
• Coordinates: 1073875..1074159 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Y82_RS15170 (L0Y82_15175)	1069523..1070944	-	1422	WP_043279886.1	FAD-binding oxidoreductase	-
L0Y82_RS15175 (L0Y82_15180)	1070963..1071358	-	396	WP_006404970.1	DUF2069 domain-containing protein	-
L0Y82_RS15180 (L0Y82_15185)	1071355..1071966	-	612	WP_006402099.1	NAD(P)H:quinone oxidoreductase	-
L0Y82_RS15185 (L0Y82_15190)	1072111..1073439	+	1329	WP_045594112.1	YihY family inner membrane protein	-
L0Y82_RS15190 (L0Y82_15195)	1073603..1073791	-	189	WP_006409259.1	hypothetical protein	-
L0Y82_RS15195 (L0Y82_15200)	1073875..1074159	-	285	WP_006409266.1	putative addiction module antidote protein	Antitoxin
L0Y82_RS15200 (L0Y82_15205)	1074163..1074456	-	294	WP_035946056.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L0Y82_RS15205 (L0Y82_15210)	1074682..1074999	-	318	WP_006402097.1	DUF962 domain-containing protein	-
L0Y82_RS15210 (L0Y82_15215)	1075160..1075615	+	456	WP_006402096.1	CBS domain-containing protein	-
L0Y82_RS15215 (L0Y82_15220)	1075764..1077698	+	1935	WP_006404968.1	MFS transporter	-
L0Y82_RS15220 (L0Y82_15225)	1077867..1078967	+	1101	WP_006404967.1	chorismate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-88)

Antitoxin

• Uncharacterized protein HI1420 (5-90)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11168.92 Da
• Isoelectric Point: 9.4615

>T231590 WP_035946056.1 NZ_CP090673:c1074456-1074163 [Burkholderia multivorans]
MVTLLRSTEFNDWLANLRDQRGKARIAARLISAQLGNFGEYRVLDEGVCELKIDFGPGYRVYYVRRGEVVYLLLCGGDKS
TQRKDIKPVQMARELKE

Nucleotide

• Length: 294 bp

>T231590 NZ_CP126932:c118738-118340 [Escherichia coli O78:H51]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9986.38 Da
• Isoelectric Point: 6.2121

>AT231590 WP_006409266.1 NZ_CP090673:c1074159-1073875 [Burkholderia multivorans]
MTNYTEFDAADYLDNDEAVAEFLAVAAEDPNPDVFLAAVAAVARARGMTKVAAAAGVGRVALHKALTPGAHPRYETVQKI
IRSLGLRMSFTVAH

Nucleotide

• Length: 285 bp

>AT231590 NZ_CP126932:c118965-118738 [Escherichia coli O78:H51]
ATGGAAACCACCGTATTTTTCAGTAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: J4SGI3