Detailed information of TA system TA231261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 486790..487480
• Replicon: plasmid pNYY1_1
• Accession: NZ_CP090580
• Organism: Methylobacterium radiotolerans strain NYY1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LZ599_RS30190
• Protein ID: WP_043074741.1
• Coordinates: 487034..487480 (+)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1M8J7
• Locus tag: LZ599_RS30185
• Protein ID: WP_012329619.1
• Coordinates: 486790..487029 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ599_RS30160 (LZ599_30160)	481857..483545	-	1689	WP_012329614.1	HAMP domain-containing methyl-accepting chemotaxis protein	-
LZ599_RS30165 (LZ599_30165)	483792..483977	+	186	WP_012329615.1	hypothetical protein	-
LZ599_RS30170 (LZ599_30170)	484134..484409	+	276	WP_024827340.1	GNAT family N-acetyltransferase	-
LZ599_RS30175 (LZ599_30175)	484485..485807	-	1323	WP_024827339.1	TAXI family TRAP transporter solute-binding subunit	-
LZ599_RS30180 (LZ599_30180)	485895..486503	-	609	WP_053620990.1	PAS domain-containing protein	-
LZ599_RS30185 (LZ599_30185)	486790..487029	+	240	WP_012329619.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LZ599_RS30190 (LZ599_30190)	487034..487480	+	447	WP_043074741.1	type II toxin-antitoxin system VapC family toxin	Toxin
LZ599_RS30195 (LZ599_30195)	487526..488137	-	612	WP_053620991.1	TetR/AcrR family transcriptional regulator	-
LZ599_RS30200 (LZ599_30200)	488431..489516	+	1086	WP_053620992.1	acetylhydrolase	-
LZ599_RS30205 (LZ599_30205)	489589..490152	-	564	WP_012329623.1	TetR/AcrR family transcriptional regulator	-
LZ599_RS30210 (LZ599_30210)	490255..491460	+	1206	WP_053620993.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..657971	657971

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-64)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 15606.04 Da
• Isoelectric Point: 6.9677

>T231261 WP_043074741.1 NZ_CP090580:487034-487480 [Methylobacterium radiotolerans]
VYLVDTNVLSAGAPTKAVPAVDLIDWMDRNSAGLHLSVVTIAEIEDGIAKSRREGAARKAERLAAWLETLLHLYAAKVLP
IDLAVARHIGGLSDLARGQGHAPGFADLAIAATARCNGWMILTRNVRHFAPLGVPACDPFVALPADGP

Nucleotide

• Length: 447 bp

>T231261 NZ_CP126226:489523-489873 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8523.78 Da
• Isoelectric Point: 7.0109

>AT231261 WP_012329619.1 NZ_CP090580:486790-487029 [Methylobacterium radiotolerans]
MQEIQLRDAKATLSAVIDQAKQGAPSIITRHGRPEAVLLSFEEWQRLSRVPSFGRLLMAAPVAADDLPSPSGGLRSAEL

Nucleotide

• Length: 240 bp

>AT231261 NZ_CP126226:489236-489517 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Antitoxin

• AlphaFold DB: B1M8J7