Detailed information of TA system TA230964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 1419796..1420401
• Replicon: chromosome
• Accession: NZ_CP090511
• Organism: Xylella fastidiosa subsp. multiplex strain Oak 35874

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: LZ754_RS06530
• Protein ID: WP_004089252.1
• Coordinates: 1419796..1420092 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LZ754_RS06535
• Protein ID: WP_038211623.1
• Coordinates: 1420096..1420401 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ754_RS06505 (LZ754_06505)	1414970..1415938	+	969	WP_004084992.1	hypothetical protein	-
LZ754_RS06510 (LZ754_06510)	1416197..1416643	+	447	WP_004084994.1	hypothetical protein	-
LZ754_RS06515 (LZ754_06515)	1416747..1417991	-	1245	WP_234499694.1	tail fiber protein	-
LZ754_RS06520 (LZ754_06520)	1417995..1418555	-	561	WP_004084996.1	DUF2612 domain-containing protein	-
LZ754_RS06525 (LZ754_06525)	1418552..1419697	-	1146	WP_234499695.1	baseplate J/gp47 family protein	-
LZ754_RS06530 (LZ754_06530)	1419796..1420092	+	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZ754_RS06535 (LZ754_06535)	1420096..1420401	+	306	WP_038211623.1	putative addiction module antidote protein	Antitoxin
LZ754_RS06540 (LZ754_06540)	1420412..1420765	-	354	WP_071870016.1	hypothetical protein	-
LZ754_RS06545 (LZ754_06545)	1420762..1421322	-	561	WP_234499696.1	Gp138 family membrane-puncturing spike protein	-
LZ754_RS06550 (LZ754_06550)	1421399..1422229	-	831	WP_027700631.1	hypothetical protein	-
LZ754_RS06555 (LZ754_06555)	1422226..1422543	-	318	WP_012382585.1	hypothetical protein	-
LZ754_RS06560 (LZ754_06560)	1422543..1423313	-	771	WP_234499697.1	hypothetical protein	-
LZ754_RS06565 (LZ754_06565)	1423424..1423651	+	228	WP_004091377.1	DUF6290 family protein	-
LZ754_RS06570 (LZ754_06570)	1423635..1423901	+	267	WP_004087087.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LZ754_RS06575 (LZ754_06575)	1423912..1424823	-	912	WP_234499698.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (50-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T230964 WP_004089252.1 NZ_CP090511:1419796-1420092 [Xylella fastidiosa subsp. multiplex]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T230964 NZ_CP125734:c3888812-3888495 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAAATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10982.42 Da
• Isoelectric Point: 5.0941

>AT230964 WP_038211623.1 NZ_CP090511:1420096-1420401 [Xylella fastidiosa subsp. multiplex]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSIDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT230964 NZ_CP125734:c3888502-3888215 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A060HBK9