Detailed information of TA system TA230514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 965557..967382
• Replicon: chromosome
• Accession: NZ_CP090378
• Organism: Vibrio cholerae strain BY369

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: LVJ30_RS04510
• Protein ID: WP_000392003.1
• Coordinates: 966021..967382 (-)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LVJ30_RS04505
• Protein ID: WP_000804849.1
• Coordinates: 965557..966024 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ30_RS04490 (LVJ30_04495)	960837..961541	-	705	WP_001880605.1	nuclease-related domain-containing protein	-
LVJ30_RS04495 (LVJ30_04500)	961611..964547	-	2937	WP_001283872.1	DEAD/DEAH box helicase family protein	-
LVJ30_RS04500 (LVJ30_04505)	964551..965129	-	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
LVJ30_RS04505 (LVJ30_04510)	965557..966024	-	468	WP_000804849.1	helix-turn-helix transcriptional regulator	Antitoxin
LVJ30_RS04510 (LVJ30_04515)	966021..967382	-	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
LVJ30_RS04515 (LVJ30_04520)	967986..968963	+	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
LVJ30_RS04520 (LVJ30_04525)	968989..969669	-	681	Protein_837	IS5 family transposase	-
LVJ30_RS04525 (LVJ30_04530)	970011..971531	-	1521	WP_000640055.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	tcpI / tcpP / tcpH / tcpA / tcpB / tcpQ / tcpC / tcpR / tcpD / tcpS / tcpT / tcpE / tcpF / tcpN/toxT / tcpJ / acfB / acfC / acfA / acfD	960837..1005678	44841
	-	flank	IS/Tn	-	-	968211..968963	752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T230514 WP_000392003.1 NZ_CP090378:c967382-966021 [Vibrio cholerae]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T230514 NZ_CP124915:34890-35165 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAATT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT230514 WP_000804849.1 NZ_CP090378:c966024-965557 [Vibrio cholerae]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT230514 NZ_CP124915:34631-34897 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8