Detailed information of TA system TA230356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4480773..4481368
• Replicon: chromosome
• Accession: NZ_CP090244
• Organism: Serratia marcescens strain SMNSF-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S6LRI1
• Locus tag: LXH15_RS21485
• Protein ID: WP_071605322.1
• Coordinates: 4481114..4481368 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LXH15_RS21480
• Protein ID: WP_223294898.1
• Coordinates: 4480773..4481117 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXH15_RS21460 (LXH15_21460)	4476281..4477096	-	816	WP_234213189.1	GPO family capsid scaffolding protein	-
LXH15_RS21465 (LXH15_21465)	4477239..4479011	+	1773	WP_234213190.1	terminase ATPase subunit family protein	-
LXH15_RS21470 (LXH15_21470)	4479011..4479697	+	687	WP_060445964.1	terminase family protein	-
LXH15_RS21475 (LXH15_21475)	4479694..4480728	+	1035	WP_033639335.1	phage portal protein	-
LXH15_RS21480 (LXH15_21480)	4480773..4481117	-	345	WP_223294898.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LXH15_RS21485 (LXH15_21485)	4481114..4481368	-	255	WP_071605322.1	type II toxin-antitoxin system HicA family toxin	Toxin
LXH15_RS21490 (LXH15_21490)	4481727..4481924	-	198	WP_126186641.1	hypothetical protein	-
LXH15_RS21495 (LXH15_21495)	4481972..4482154	-	183	WP_230202221.1	hypothetical protein	-
LXH15_RS21500 (LXH15_21500)	4482235..4484451	-	2217	WP_234213191.1	replication endonuclease	-
LXH15_RS21505 (LXH15_21505)	4484448..4484846	-	399	WP_234213192.1	hypothetical protein	-
LXH15_RS21510 (LXH15_21510)	4484967..4485191	-	225	WP_234213193.1	TraR/DksA C4-type zinc finger protein	-
LXH15_RS21515 (LXH15_21515)	4485191..4485502	-	312	WP_234213194.1	DUF5405 family protein	-
LXH15_RS21520 (LXH15_21520)	4485502..4485732	-	231	WP_234213195.1	DUF2732 family protein	-
LXH15_RS21525 (LXH15_21525)	4485796..4486098	-	303	WP_031221632.1	hypothetical protein	-
LXH15_RS21530 (LXH15_21530)	4486110..4486289	-	180	WP_048322123.1	DUF2724 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4446358..4490149	43791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (56-101)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9495.97 Da
• Isoelectric Point: 11.1073

>T230356 WP_071605322.1 NZ_CP090244:c4481368-4481114 [Serratia marcescens]
MNKRHQKTLSDVFARPVNGSIKWSDIEALFTALGAEIHEREGSRIAVLLKGEKRVFHRPHPRPTTDKGAVNSIRIWLDSL
GIKP

Nucleotide

• Length: 255 bp

>T230356 NZ_CP124882:2842130-2842273 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAAGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12531.10 Da
• Isoelectric Point: 4.5789

>AT230356 WP_223294898.1 NZ_CP090244:c4481117-4480773 [Serratia marcescens]
MMNNTLKIDGHTAVINFDPEIEMFRGEFVGLNGGADFYAYSVDELKKEGAISLAVFLDECHKDGIEPYKSYSGKVTTRLS
PERHQALAIAAQATGQSINELLNEGVDLVIEKHS

Nucleotide

• Length: 345 bp

>AT230356 NZ_CP124882:c2842467-2842205 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTATA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATACCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTCTTCTTTAACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Structures

Toxin

• AlphaFold DB: A0A1S6LRI1