Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4931686..4932328 Replicon chromosome
Accession NZ_CP090081
Organism Bacillus cereus strain CPT56D-587-MTF

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag LW858_RS25385 Protein ID WP_000635963.1
Coordinates 4931686..4932036 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag LW858_RS25390 Protein ID WP_000004570.1
Coordinates 4932041..4932328 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LW858_RS25365 (LW858_25365) 4927037..4927495 - 459 WP_233935600.1 SprT family protein -
LW858_RS25370 (LW858_25370) 4927691..4927807 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
LW858_RS25375 (LW858_25375) 4927865..4930033 - 2169 WP_233935602.1 Tex family protein -
LW858_RS25380 (LW858_25380) 4930334..4931521 + 1188 WP_078187060.1 IS110 family transposase -
LW858_RS25385 (LW858_25385) 4931686..4932036 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LW858_RS25390 (LW858_25390) 4932041..4932328 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
LW858_RS25395 (LW858_25395) 4932637..4933806 - 1170 WP_233935604.1 alanine racemase -
LW858_RS25400 (LW858_25400) 4933998..4935401 - 1404 WP_233935200.1 IS66 family transposase -
LW858_RS25405 (LW858_25405) 4935555..4936505 - 951 WP_233935605.1 outer membrane lipoprotein carrier protein LolA -
LW858_RS25410 (LW858_25410) 4936662..4937021 - 360 WP_000583416.1 holo-ACP synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4930334..4931521 1187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T230023 WP_000635963.1 NZ_CP090081:c4932036-4931686 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T230023 NZ_CP124662:c5843697-5843407 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCTCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTTTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT230023 WP_000004570.1 NZ_CP090081:c4932328-4932041 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT230023 NZ_CP124662:c5843993-5843694 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References