Detailed information of TA system TA230007

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 917947..918604
• Replicon: chromosome
• Accession: NZ_CP090078
• Organism: Klebsiella michiganensis strain KM41

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: J5U333
• Locus tag: LW125_RS04485
• Protein ID: WP_004854060.1
• Coordinates: 918194..918604 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: LW125_RS04480
• Protein ID: WP_220152228.1
• Coordinates: 917947..918213 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LW125_RS04465	913199..913624	-	426	WP_220152227.1	PTS sugar transporter subunit IIA	-
LW125_RS04470	913745..916543	-	2799	WP_154062421.1	transcriptional regulator DagR	-
LW125_RS04475	916719..917702	-	984	WP_004854063.1	tRNA-modifying protein YgfZ	-
LW125_RS04480	917947..918213	+	267	WP_220152228.1	FAD assembly factor SdhE	Antitoxin
LW125_RS04485	918194..918604	+	411	WP_004854060.1	protein YgfX	Toxin
LW125_RS04490	918613..919134	-	522	WP_009654974.1	flavodoxin FldB	-
LW125_RS04495	919256..920152	+	897	WP_139531212.1	site-specific tyrosine recombinase XerD	-
LW125_RS04500	920175..920888	+	714	WP_004854054.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
LW125_RS04505	920894..922627	+	1734	WP_004854053.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16106.97 Da
• Isoelectric Point: 10.9455

>T230007 WP_004854060.1 NZ_CP090078:918194-918604 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Nucleotide

• Length: 411 bp

>T230007 NZ_CP124659:c131836-131459 [Pseudomonas aeruginosa]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10469.92 Da
• Isoelectric Point: 4.8616

>AT230007 WP_220152228.1 NZ_CP090078:917947-918213 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDDDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT230007 NZ_CP124659:c131478-131143 [Pseudomonas aeruginosa]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GYA5