Detailed information of TA system TA229821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-couple_hipB
• Location: 87383..87990
• Replicon: plasmid pORIO3
• Accession: NZ_CP089969
• Organism: Cereibacter azotoformans strain ORIO

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ORIO_RS22365
• Protein ID: WP_238823969.1
• Coordinates: 87383..87634 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ORIO_RS22370
• Protein ID: WP_238823971.1
• Coordinates: 87646..87990 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ORIO_RS22340 (ORIO_22340)	82501..82772	-	272	Protein_95	BrnT family toxin	-
ORIO_RS22345 (ORIO_22345)	83425..84450	+	1026	WP_238823968.1	hypothetical protein	-
ORIO_RS22350 (ORIO_22350)	84766..84996	+	231	Protein_97	integrase	-
ORIO_RS22355 (ORIO_22355)	85174..86819	+	1646	Protein_98	IS1634 family transposase	-
ORIO_RS22360 (ORIO_22360)	86894..87298	+	405	Protein_99	tyrosine-type recombinase/integrase	-
ORIO_RS22365 (ORIO_22365)	87383..87634	+	252	WP_238823969.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ORIO_RS22370 (ORIO_22370)	87646..87990	+	345	WP_238823971.1	helix-turn-helix transcriptional regulator	Antitoxin
ORIO_RS22375 (ORIO_22375)	88411..91749	-	3339	WP_238823972.1	error-prone DNA polymerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..136759	136759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-79)

Antitoxin

• Cro/C1-type helix-turn-helix domain (58-112)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9325.84 Da
• Isoelectric Point: 11.2302

>T229821 WP_238823969.1 NZ_CP089969:87383-87634 [Cereibacter azotoformans]
MKQISYTKAALRTLRRMPANTAALIRSKIEDYAQAPASQANNVKSLKGREGIRLRVRDWRVIMDDRGNVLAVLEIGPRGG
IYD

Nucleotide

• Length: 252 bp

>T229821 NZ_CP124527:24346-24636 [Psychrobacter sp. WB2]
ATGGACGTTGTGAACTACACTGATTTTAGAAAGAGCTTAGCAAAGTATATCGATAAGGTAGACACTGATAAAGGCCCGAT
ATTAATTACAAGACAAAATGCTAAACCGACGGTATTAATGAGTTTAGAAGAGTTTAACTCGTATGAGGAAACACTGCATC
TTTTAAGCAGCCCTACCAATGCCAAGCGTCTCAGACGCTCTATTGCTGATGCTAAAGCTGGTCGCTTGATTGAACGTGAG
ATTGATGCCAAAGATATGCCACTCACTTTTGATGATGAGGTAGACGCTTGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12290.91 Da
• Isoelectric Point: 4.5778

>AT229821 WP_238823971.1 NZ_CP089969:87646-87990 [Cereibacter azotoformans]
MNDMVTIPREEYDRLKTAAEDLADLQSYDRMKAALASGDDELIPADFANRLLNGESPLRVYRDLRGLTQAALAGKAGVNR
VTVAEIETGRKKGSIATLRALAGALGVTLDDLTE

Nucleotide

• Length: 345 bp

>AT229821 NZ_CP124527:24633-24887 [Psychrobacter sp. WB2]
TTGATACTATCTTGGACGGAAGACGCGTGGGAGGACTATGAGTACTGGCAAAGAACCAGTAAAGAGAAAGTCAAACAGAT
CGGTAAGCTGATTAAAGCCATCAAACGTGACCCATTTAAGGGTATTGGCAAACCTGAACCACTCAAACATGACCTTGCAG
GCTACTGGTCTCGTCGAATTGATCAAGAGCATCGGCTGGTTTATGAAATTCAAGATGATGCGATCATTATCGTCCAGTGC
CGTTATCATTACTAA