229591

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 25052..25574 Replicon plasmid pKP059
Accession NZ_CP089883
Organism Klebsiella pneumoniae strain KP059

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag LVQ69_RS25870 Protein ID WP_004181778.1
Coordinates 25052..25336 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag LVQ69_RS25875 Protein ID WP_004181777.1
Coordinates 25326..25574 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVQ69_RS25835 (LVQ69_25800) 20382..21569 - 1188 WP_040209642.1 RNA-guided endonuclease TnpB family protein -
LVQ69_RS25840 (LVQ69_25805) 21605..22033 + 429 WP_077257669.1 IS200/IS605 family transposase -
LVQ69_RS25845 (LVQ69_25810) 22112..22336 + 225 Protein_18 transposase -
LVQ69_RS25850 (LVQ69_25815) 22607..23233 - 627 Protein_19 transposase -
LVQ69_RS25855 (LVQ69_25820) 23286..24254 - 969 WP_167835278.1 IS5 family transposase -
LVQ69_RS25860 (LVQ69_25825) 24272..24898 - 627 WP_235353074.1 RNA-guided endonuclease TnpB family protein -
LVQ69_RS25865 (LVQ69_25830) 24934..25035 + 102 Protein_22 IS200/IS605 family transposase -
LVQ69_RS25870 (LVQ69_25835) 25052..25336 - 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVQ69_RS25875 (LVQ69_25840) 25326..25574 - 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LVQ69_RS25880 (LVQ69_25845) 25865..27664 + 1800 WP_004181776.1 ATP-dependent helicase -
LVQ69_RS25885 (LVQ69_25850) 27998..28984 + 987 WP_025368599.1 hypothetical protein -
LVQ69_RS25890 (LVQ69_25855) 29129..29482 + 354 WP_004181774.1 hypothetical protein -
LVQ69_RS25895 (LVQ69_25860) 29544..30365 + 822 WP_004181772.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..262094 262094


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T229591 WP_004181778.1 NZ_CP089883:c25336-25052 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T229591 NZ_CP124495:c4005155-4004841 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT229591 WP_004181777.1 NZ_CP089883:c25574-25326 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT229591 NZ_CP124495:c4005391-4005158 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References