Detailed information of TA system TA229432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4608677..4609431
• Replicon: chromosome
• Accession: NZ_CP089797
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain ZTA19/00790

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: LUY11_RS22240
• Protein ID: WP_000558166.1
• Coordinates: 4608677..4608988 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LUY11_RS22245
• Protein ID: WP_001259011.1
• Coordinates: 4608985..4609431 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUY11_RS22210 (4604335)	4604335..4605237	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
LUY11_RS22215 (4605234)	4605234..4605869	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
LUY11_RS22220 (4605866)	4605866..4606795	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
LUY11_RS22225 (4606842)	4606842..4607132	-	291	WP_000362052.1	DNA-binding transcriptional regulator	-
LUY11_RS22230 (4607133)	4607133..4607444	-	312	WP_023138876.1	hypothetical protein	-
LUY11_RS22235 (4607662)	4607662..4608591	+	930	WP_001127698.1	alpha/beta hydrolase	-
LUY11_RS22240 (4608677)	4608677..4608988	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
LUY11_RS22245 (4608985)	4608985..4609431	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
LUY11_RS22250 (4609446)	4609446..4610387	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
LUY11_RS22255 (4610432)	4610432..4610869	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
LUY11_RS22260 (4610866)	4610866..4611738	-	873	WP_000921423.1	virulence factor BrkB family protein	-
LUY11_RS22265 (4611732)	4611732..4612331	-	600	WP_000965695.1	glucose-1-phosphatase	-
LUY11_RS22270 (4612522)	4612522..4613325	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
LUY11_RS22275 (4613359)	4613359..4614255	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T229432 WP_000558166.1 NZ_CP089797:4608677-4608988 [Salmonella enterica subsp. enterica serovar Kentucky]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T229432 NZ_CP124471:c4036330-4036016 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT229432 WP_001259011.1 NZ_CP089797:4608985-4609431 [Salmonella enterica subsp. enterica serovar Kentucky]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT229432 NZ_CP124471:c4036566-4036333 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A0R9ME90