Detailed information of TA system TA229350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 299191..299982
• Replicon: chromosome
• Accession: NZ_CP089792
• Organism: Salmonella enterica subsp. enterica serovar Kentucky strain ZTA19/00816

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: C0Q119
• Locus tag: LUY16_RS01385
• Protein ID: WP_000369560.1
• Coordinates: 299464..299982 (+)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q57IR1
• Locus tag: LUY16_RS01380
• Protein ID: WP_001275017.1
• Coordinates: 299191..299460 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUY16_RS01360 (294772)	294772..295440	+	669	WP_000617729.1	cell division ATP-binding protein FtsE	-
LUY16_RS01365 (295433)	295433..296488	+	1056	WP_001081707.1	permease-like cell division protein FtsX	-
LUY16_RS01370 (296734)	296734..297588	+	855	WP_000159621.1	RNA polymerase sigma factor RpoH	-
LUY16_RS01375 (297910)	297910..299013	+	1104	WP_001533929.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
LUY16_RS01380 (299191)	299191..299460	+	270	WP_001275017.1	DUF1778 domain-containing protein	Antitoxin
LUY16_RS01385 (299464)	299464..299982	+	519	WP_000369560.1	GNAT family N-acetyltransferase	Toxin
LUY16_RS01390 (299979)	299979..300362	-	384	WP_000778873.1	aspartate 1-decarboxylase autocleavage activator PanM	-
LUY16_RS01395 (300784)	300784..301893	+	1110	WP_023224165.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LUY16_RS01400 (301953)	301953..302879	+	927	WP_000003005.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LUY16_RS01405 (302876)	302876..304153	+	1278	WP_000803766.1	branched chain amino acid ABC transporter permease LivM	-
LUY16_RS01410 (304150)	304150..304917	+	768	WP_000082083.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 19414.16 Da
• Isoelectric Point: 8.9619

>T229350 WP_000369560.1 NZ_CP089792:299464-299982 [Salmonella enterica subsp. enterica serovar Kentucky]
VDNLTIEILADNAEYNWRQFDCGEASLNLFLTQHLQRQHNNKILRGYVLRTTTPERRVLGYYTLSGSCFERASLPSRTQQ
KRIPYQNIPSVTLGRLAVDLSLQGKGWGAILVTHAMKVVWSASLAVGIHGIFVEALNEKAQAFYQRLGFISLSGENEHAL
FYPTKSIEQLFG

Nucleotide

• Length: 519 bp

>T229350 NZ_CP124455:1234616-1234918 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10086.53 Da
• Isoelectric Point: 7.2056

>AT229350 WP_001275017.1 NZ_CP089792:299191-299460 [Salmonella enterica subsp. enterica serovar Kentucky]
MSALKKQRIDLRLTDGDKSMIEEAAAITNQSITQFMLNSAAERAAEVLEQHRRVILNEASWARVMDALSHPPTPNEKLKR
AAQRLQDME

Nucleotide

• Length: 270 bp

>AT229350 NZ_CP124455:1234954-1235295 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A5H5BA59

Antitoxin

• AlphaFold DB: A0A5I8URP5