Detailed information of TA system TA229247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2841475..2842115
• Replicon: chromosome
• Accession: NZ_CP089781
• Organism: Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: LVJ76_RS13320
• Protein ID: WP_003412970.1
• Coordinates: 2841475..2841894 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: LVJ76_RS13325
• Protein ID: WP_003412975.1
• Coordinates: 2841891..2842115 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ76_RS13290 (LVJ76_13300)	2837060..2837782	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
LVJ76_RS13295 (LVJ76_13305)	2838299..2838526	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ76_RS13300 (LVJ76_13310)	2838523..2838924	+	402	WP_003412963.1	PIN domain-containing protein	-
LVJ76_RS13305 (LVJ76_13315)	2838959..2839879	-	921	WP_003412965.1	restriction endonuclease	-
LVJ76_RS13310 (LVJ76_13320)	2840220..2840465	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LVJ76_RS13315 (LVJ76_13325)	2840524..2841474	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
LVJ76_RS13320 (LVJ76_13330)	2841475..2841894	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ76_RS13325 (LVJ76_13335)	2841891..2842115	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
LVJ76_RS13330 (LVJ76_13340)	2842146..2844989	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
LVJ76_RS13335 (LVJ76_13345)	2845061..2845462	-	402	WP_003412981.1	hypothetical protein	-
LVJ76_RS13340 (LVJ76_13350)	2845462..2845932	-	471	WP_003899364.1	transcription antitermination factor NusB	-
LVJ76_RS13345 (LVJ76_13355)	2845935..2846498	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T229247 WP_003412970.1 NZ_CP089781:c2841894-2841475 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T229247 NZ_CP124424:c790422-790171 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT229247 WP_003412975.1 NZ_CP089781:c2842115-2841891 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT229247 NZ_CP124424:c790174-789920 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09