Detailed information of TA system TA229235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2179324..2180027
• Replicon: chromosome
• Accession: NZ_CP089781
• Organism: Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: LVJ76_RS10130
• Protein ID: WP_003409778.1
• Coordinates: 2179324..2179653 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: LVJ76_RS10135
• Protein ID: WP_003409780.1
• Coordinates: 2179650..2180027 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ76_RS10110 (LVJ76_10125)	2175707..2176777	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
LVJ76_RS10115 (LVJ76_10130)	2176774..2177289	+	516	WP_003409718.1	flavin reductase family protein	-
LVJ76_RS10120 (LVJ76_10135)	2177286..2178347	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
LVJ76_RS10125 (LVJ76_10140)	2178344..2179114	+	771	WP_003409775.1	SDR family oxidoreductase	-
LVJ76_RS10130 (LVJ76_10145)	2179324..2179653	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LVJ76_RS10135 (LVJ76_10150)	2179650..2180027	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
LVJ76_RS10140 (LVJ76_10155)	2180024..2180614	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
LVJ76_RS10145 (LVJ76_10160)	2180669..2182033	+	1365	WP_014585532.1	HNH endonuclease signature motif containing protein	-
LVJ76_RS10150 (LVJ76_10165)	2182188..2182640	-	453	WP_003899095.1	lipoprotein	-
LVJ76_RS10155 (LVJ76_10170)	2182704..2183105	+	402	WP_003409869.1	hypothetical protein	-
LVJ76_RS10160 (LVJ76_10175)	2183098..2183280	-	183	WP_003409870.1	hypothetical protein	-
LVJ76_RS10165 (LVJ76_10180)	2183394..2183744	-	351	WP_003899096.1	hypothetical protein	-
LVJ76_RS10170 (LVJ76_10185)	2183755..2184657	-	903	WP_003899097.1	hypothetical protein	-
LVJ76_RS10175 (LVJ76_10190)	2184678..2184869	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T229235 WP_003409778.1 NZ_CP089781:c2179653-2179324 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T229235 NZ_CP124420:4001817-4002104 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT229235 WP_003409780.1 NZ_CP089781:c2180027-2179650 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT229235 NZ_CP124420:4002091-4002393 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8