Detailed information of TA system TA229228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1385954..1386513
• Replicon: chromosome
• Accession: NZ_CP089781
• Organism: Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: LVJ76_RS06630
• Protein ID: WP_003898789.1
• Coordinates: 1385954..1386247 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: LVJ76_RS06635
• Protein ID: WP_003406322.1
• Coordinates: 1386244..1386513 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ76_RS06605 (LVJ76_06605)	1381547..1381807	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
LVJ76_RS06610 (LVJ76_06610)	1381804..1382235	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
LVJ76_RS06615 (LVJ76_06615)	1382258..1383946	-	1689	WP_003910308.1	PE family protein	-
LVJ76_RS06620 (LVJ76_06620)	1384126..1384986	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ76_RS06625 (LVJ76_06625)	1385067..1385897	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ76_RS06630 (LVJ76_06630)	1385954..1386247	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
LVJ76_RS06635 (LVJ76_06635)	1386244..1386513	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ76_RS06640 (LVJ76_06640)	1386626..1390321	-	3696	WP_009938349.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
LVJ76_RS06645 (LVJ76_06645)	1390463..1391251	-	789	WP_003406325.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T229228 WP_003898789.1 NZ_CP089781:c1386247-1385954 [Mycobacterium tuberculosis]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T229228 NZ_CP124420:c2888145-2887618 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT229228 WP_003406322.1 NZ_CP089781:c1386513-1386244 [Mycobacterium tuberculosis]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT229228 NZ_CP124420:c2888418-2888152 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2