Detailed information of TA system TA229209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 636510..637186
• Replicon: chromosome
• Accession: NZ_CP089781
• Organism: Mycobacterium tuberculosis strain 02-R1179

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: LVJ76_RS02880
• Protein ID: WP_003898500.1
• Coordinates: 636510..636923 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: LVJ76_RS02885
• Protein ID: WP_003402915.1
• Coordinates: 636920..637186 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ76_RS02850 (LVJ76_02855)	631855..632157	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
LVJ76_RS02855 (LVJ76_02860)	632217..632495	-	279	WP_003402901.1	hypothetical protein	-
LVJ76_RS02860 (LVJ76_02865)	632492..633745	-	1254	WP_003402904.1	inorganic phosphate transporter	-
LVJ76_RS02865 (LVJ76_02870)	633865..634251	-	387	WP_003402907.1	VOC family protein	-
LVJ76_RS02870 (LVJ76_02875)	634314..635198	-	885	WP_003402909.1	SDR family oxidoreductase	-
LVJ76_RS02875 (LVJ76_02880)	635294..636196	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
LVJ76_RS02880 (LVJ76_02885)	636510..636923	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ76_RS02885 (LVJ76_02890)	636920..637186	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LVJ76_RS02890 (LVJ76_02895)	637378..639093	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
LVJ76_RS02895 (LVJ76_02900)	639171..640775	+	1605	WP_003402920.1	amidohydrolase	-
LVJ76_RS02900 (LVJ76_02905)	640772..641752	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T229209 WP_003898500.1 NZ_CP089781:c636923-636510 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T229209 NZ_CP124415:3598383-3598667 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT229209 WP_003402915.1 NZ_CP089781:c637186-636920 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT229209 NZ_CP124415:3598147-3598380 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0