Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3066002..3066681 Replicon chromosome
Accession NZ_CP089780
Organism Mycobacterium tuberculosis strain M0003941-3

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag LVJ75_RS14550 Protein ID WP_003414059.1
Coordinates 3066002..3066418 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag LVJ75_RS14555 Protein ID WP_003414061.1
Coordinates 3066415..3066681 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ75_RS14530 (LVJ75_14515) 3062054..3062956 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
LVJ75_RS14535 (LVJ75_14520) 3063025..3063777 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
LVJ75_RS14540 (LVJ75_14530) 3064021..3064296 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
LVJ75_RS14545 (LVJ75_14535) 3064293..3065915 - 1623 WP_003414057.1 class I SAM-dependent DNA methyltransferase -
LVJ75_RS14550 (LVJ75_14540) 3066002..3066418 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
LVJ75_RS14555 (LVJ75_14545) 3066415..3066681 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ75_RS14560 (LVJ75_14550) 3066707..3067102 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
LVJ75_RS14565 (LVJ75_14555) 3067099..3067368 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ75_RS14570 (LVJ75_14560) 3067378..3068472 - 1095 WP_003414068.1 restriction endonuclease subunit S -
LVJ75_RS14575 (LVJ75_14565) 3068469..3068888 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
LVJ75_RS14580 (LVJ75_14570) 3068887..3068961 + 75 Protein_2877 hypothetical protein -
LVJ75_RS14585 (LVJ75_14575) 3068962..3069441 - 480 WP_003414073.1 dihydrofolate reductase -
LVJ75_RS14590 (LVJ75_14580) 3069512..3070312 - 801 WP_003911953.1 thymidylate synthase -
LVJ75_RS14595 (LVJ75_14585) 3070468..3071205 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T229192 WP_003414059.1 NZ_CP089780:c3066418-3066002 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T229192 NZ_CP124415:c238627-237305 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCAGCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT229192 WP_003414061.1 NZ_CP089780:c3066681-3066415 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT229192 NZ_CP124415:c238893-238627 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5SV2

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References