Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2265520..2266146 Replicon chromosome
Accession NZ_CP089778
Organism Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag LVJ73_RS10605 Protein ID WP_003410075.1
Coordinates 2265748..2266146 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag LVJ73_RS10600 Protein ID WP_003911750.1
Coordinates 2265520..2265747 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ73_RS10590 (LVJ73_10605) 2263559..2263903 - 345 WP_003410065.1 ferredoxin family protein -
LVJ73_RS10595 (LVJ73_10610) 2264092..2265261 - 1170 WP_003899126.1 ATP-binding protein -
LVJ73_RS10600 (LVJ73_10615) 2265520..2265747 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ73_RS10605 (LVJ73_10620) 2265748..2266146 + 399 WP_003410075.1 PIN domain nuclease Toxin
LVJ73_RS10610 (LVJ73_10625) 2266329..2266760 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
LVJ73_RS10615 (LVJ73_10630) 2266861..2267295 + 435 WP_003914358.1 DUF1398 domain-containing protein -
LVJ73_RS10620 (LVJ73_10635) 2267732..2267911 - 180 Protein_2099 hypothetical protein -
LVJ73_RS10625 (LVJ73_10640) 2267999..2268619 + 621 WP_003410086.1 IS110 family transposase -
LVJ73_RS10630 (LVJ73_10645) 2268573..2269163 + 591 WP_003899131.1 IS110 family transposase -
LVJ73_RS10635 (LVJ73_10650) 2269291..2270547 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T229060 WP_003410075.1 NZ_CP089778:2265748-2266146 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T229060 NZ_CP124375:c68584-68168 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT229060 WP_003911750.1 NZ_CP089778:2265520-2265747 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT229060 NZ_CP124375:c68811-68581 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References