Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2232888..2233576 Replicon chromosome
Accession NZ_CP089778
Organism Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)


Gene name vapC Uniprot ID P9WF64
Locus tag LVJ73_RS10435 Protein ID WP_003899117.1
Coordinates 2232888..2233307 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag LVJ73_RS10440 Protein ID WP_003409968.1
Coordinates 2233316..2233576 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ73_RS10415 (LVJ73_10430) 2228383..2229231 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
LVJ73_RS10420 (LVJ73_10435) 2229194..2230639 - 1446 WP_003899115.1 APC family permease -
LVJ73_RS10425 (LVJ73_10440) 2230818..2231504 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
LVJ73_RS10430 (LVJ73_10445) 2231695..2232663 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
LVJ73_RS10435 (LVJ73_10450) 2232888..2233307 - 420 WP_003899117.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ73_RS10440 (LVJ73_10455) 2233316..2233576 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ73_RS10445 (LVJ73_10460) 2233719..2235395 + 1677 WP_070889706.1 PecA family PE domain-processing aspartic protease -
LVJ73_RS10450 (LVJ73_10465) 2235383..2236036 - 654 WP_003409976.1 cutinase Cfp21 -
LVJ73_RS10455 (LVJ73_10470) 2236151..2236381 + 231 WP_003409980.1 DUF167 domain-containing protein -
LVJ73_RS10460 (LVJ73_10475) 2236466..2237377 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
LVJ73_RS10465 (LVJ73_10480) 2237486..2238085 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14748.87 Da        Isoelectric Point: 7.9722

>T229057 WP_003899117.1 NZ_CP089778:c2233307-2232888 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDHEFRGVVGYWPGVA

Download         Length: 420 bp

>T229057 NZ_CP124374:5121143-5121454 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT229057 WP_003409968.1 NZ_CP089778:c2233576-2233316 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT229057 NZ_CP124374:5121455-5121745 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E7TLI0

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References