Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 1968228..1968688 Replicon chromosome
Accession NZ_CP089778
Organism Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)


Gene name vapC Uniprot ID G0TKL9
Locus tag LVJ73_RS09215 Protein ID WP_003898996.1
Coordinates 1968440..1968688 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ30
Locus tag LVJ73_RS09210 Protein ID WP_003408528.1
Coordinates 1968228..1968440 (+) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ73_RS09195 (LVJ73_09205) 1964706..1965893 - 1188 WP_003898992.1 nitrate transporter NarK -
LVJ73_RS09200 (LVJ73_09210) 1966180..1966464 + 285 WP_003408522.1 DUF1876 domain-containing protein -
LVJ73_RS09205 (LVJ73_09215) 1966478..1968160 - 1683 WP_003898994.1 SulP family inorganic anion transporter -
LVJ73_RS09210 (LVJ73_09220) 1968228..1968440 + 213 WP_003408528.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ73_RS09215 (LVJ73_09225) 1968440..1968688 + 249 WP_003898996.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ73_RS09220 (LVJ73_09230) 1968696..1969433 + 738 WP_003898997.1 hypothetical protein -
LVJ73_RS09225 (LVJ73_09235) 1969527..1971227 + 1701 WP_003898998.1 serine/threonine protein kinase PknE -
LVJ73_RS09230 (LVJ73_09240) 1971512..1971913 - 402 WP_003408538.1 hypothetical protein -
LVJ73_RS09235 (LVJ73_09245) 1971903..1972514 - 612 WP_003898999.1 isopentenyl-diphosphate Delta-isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Antitoxin VapB-like

(2-69)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 8884.14 Da        Isoelectric Point: 4.1074

>T229053 WP_003898996.1 NZ_CP089778:1968440-1968688 [Mycobacterium tuberculosis]
MVIDTSALVAMLNDEPEAQRFEIAVAADHVWLMSTASYPEMATVIETRFGEPGGREPKVSGQPLLYKGDDFACIDIRAVL
AG

Download         Length: 249 bp

>T229053 NZ_CP124374:c4555672-4555145 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 7607.80 Da        Isoelectric Point: 9.3146

>AT229053 WP_003408528.1 NZ_CP089778:1968228-1968440 [Mycobacterium tuberculosis]
MELAARMGETLTQAVVVAVREQLARRTGRTRSISLREELAAIGRRCAALPVLDTRAADTILGYDERGLPA

Download         Length: 213 bp

>AT229053 NZ_CP124374:c4555945-4555679 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TKL9

Antitoxin

Source ID Structure
AlphaFold DB A0A829CFE8

References