Detailed information of TA system TA022901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 119993..121644
• Replicon: chromosome
• Accession: NC_010803
• Organism: Chlorobium limicola DSM 245

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: B3EE80
• Locus tag: CLIM_RS00570
• Protein ID: WP_012465095.1
• Coordinates: 119993..121300 (-)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: CLIM_RS00575
• Protein ID: WP_012465096.1
• Coordinates: 121297..121644 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLIM_RS00560	118357..119094	-	738	WP_190275089.1	RES family NAD+ phosphorylase	-
CLIM_RS00565	119054..119434	-	381	WP_012465094.1	MbcA/ParS/Xre antitoxin family protein	-
CLIM_RS12835	119619..119828	-	210	Protein_106	PhzF family phenazine biosynthesis protein	-
CLIM_RS13615	119850..119996	-	147	WP_190275090.1	hypothetical protein	-
CLIM_RS00570	119993..121300	-	1308	WP_012465095.1	type II toxin-antitoxin system HipA family toxin	Toxin
CLIM_RS00575	121297..121644	-	348	WP_012465096.1	helix-turn-helix transcriptional regulator	Antitoxin
CLIM_RS00580	121989..122258	+	270	WP_012465097.1	hypothetical protein	-
CLIM_RS00585	122246..122536	+	291	WP_150081502.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CLIM_RS00590	123002..123396	+	395	Protein_112	SocA family protein	-
CLIM_RS00595	123483..124580	-	1098	WP_012465099.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
CLIM_RS00600	124577..125359	-	783	WP_012465100.1	hypothetical protein	-
CLIM_RS12670	125664..125873	+	210	WP_012465101.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-116)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-68)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 48358.66 Da
• Isoelectric Point: 6.9664

>T22901 WP_012465095.1 NC_010803:c121300-119993 [Chlorobium limicola DSM 245]
VSTTARVNLWGRTIGAVSLDSDAATATFEYDPAFARSGIEIAPLTMPLSGQLYSFPSLRPETFHGLPGLLADSLPDRFGN
TLIDTWLARSGRTSGSFNAIERLCYTGSRGMGVLEYAPAIQLGGSGSAPLEIERLVELASEVLTHRNDLQVWFLDGGKEL
ALGEILRVGTSAGGARAKAVIAWNPETDEVRSGQVKAGKGFEYWLLKFDGVSGNKDREQEDPKGYGAIEHAYYRMALDAG
ITMTPCRLFEENGRRHFMTKRFDRLEDGGKLHMQSLCGMAHYDFNRAGAYGYEQALQVIRRLGLPMASVEEQFRRMVFNI
VARNQDDHVKNIAFLMDRSGNWSLAPAFDMTWSYQPGGAWTSTHQMTMNGKRSGFLPDDFRACAKSASMKRGRAETIVAE
VQDVVRRWHDYAEESRITPRQRDTIATTLRLEGFV

Nucleotide

• Length: 1308 bp

>T22901 NC_010803:c121300-119993 [Chlorobium limicola DSM 245]
GTGAGTACAACAGCAAGGGTAAACCTGTGGGGGCGCACGATCGGAGCGGTATCGCTCGATAGCGACGCTGCGACTGCAAC
CTTCGAGTACGATCCGGCCTTCGCTCGGAGCGGCATCGAAATCGCCCCGCTGACCATGCCGCTCTCCGGTCAGCTCTACT
CCTTTCCCTCGCTGCGTCCCGAAACCTTCCACGGGCTTCCGGGACTGTTGGCGGATTCGCTGCCGGACCGGTTCGGCAAT
ACGCTGATCGATACCTGGCTGGCCCGTTCCGGTCGCACGTCCGGCTCCTTCAATGCCATCGAGAGGCTCTGTTATACAGG
GTCTCGGGGCATGGGCGTTCTTGAATATGCTCCAGCCATACAATTGGGGGGCTCCGGCTCTGCACCGCTCGAAATCGAAC
GGTTGGTCGAATTGGCTTCGGAGGTGTTGACCCATCGCAACGATCTGCAGGTCTGGTTCCTCGATGGGGGCAAGGAGCTT
GCGCTCGGGGAGATTCTCCGGGTCGGCACCTCCGCGGGCGGAGCAAGAGCCAAGGCGGTAATTGCCTGGAACCCGGAAAC
CGACGAAGTCCGTTCAGGCCAGGTGAAGGCCGGAAAAGGGTTCGAGTACTGGCTGCTCAAGTTCGACGGAGTGAGCGGCA
ACAAGGACAGGGAACAGGAAGATCCAAAAGGGTACGGTGCAATCGAGCACGCATACTACCGCATGGCGCTGGATGCGGGA
ATCACCATGACGCCCTGCCGCCTGTTCGAGGAAAACGGTCGTCGCCATTTTATGACGAAGCGCTTCGACCGGTTGGAGGA
TGGAGGCAAACTGCACATGCAGTCGCTCTGCGGCATGGCGCATTACGACTTCAATCGGGCGGGAGCTTACGGGTATGAAC
AGGCGTTGCAGGTCATCAGGCGCCTTGGTTTGCCGATGGCTTCCGTGGAGGAGCAGTTCCGGCGAATGGTGTTCAATATC
GTGGCCCGCAACCAGGATGACCATGTGAAGAACATTGCCTTTCTGATGGACAGGTCGGGCAACTGGTCGCTTGCGCCAGC
GTTCGACATGACCTGGAGCTATCAACCGGGGGGAGCGTGGACATCGACCCATCAGATGACGATGAACGGCAAACGGAGCG
GATTCCTGCCGGACGACTTCAGGGCATGTGCGAAAAGCGCATCCATGAAACGCGGGCGAGCCGAAACCATCGTCGCTGAA
GTGCAGGACGTTGTTCGCAGATGGCATGATTATGCCGAGGAGTCGCGCATCACTCCCCGACAACGGGATACGATTGCAAC
AACGCTGAGACTGGAGGGCTTTGTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 12734.85 Da
• Isoelectric Point: 10.2443

>AT22901 WP_012465096.1 NC_010803:c121644-121297 [Chlorobium limicola DSM 245]
MKIEGTLTDEAILGELGCRLAQRRLELQLSQGALAEQAGVSKRTVERVEAGATTQMSSMIRVMRVLGLLEQLEALVPEAG
PRPLELLKLKGKARKRARTKQKPAEEKPWTWGDES

Nucleotide

• Length: 348 bp

>AT22901 NC_010803:c121644-121297 [Chlorobium limicola DSM 245]
ATGAAAATTGAAGGCACATTGACGGATGAGGCGATTCTCGGGGAGCTGGGCTGTCGGCTGGCGCAGCGTCGGCTGGAGCT
TCAGTTGAGTCAGGGAGCGCTTGCCGAGCAGGCTGGCGTATCGAAGCGGACGGTGGAGCGTGTCGAGGCCGGGGCTACGA
CGCAGATGTCGTCCATGATACGGGTTATGCGGGTATTGGGGCTGCTGGAACAGCTGGAGGCTCTGGTGCCGGAGGCGGGG
CCGCGGCCGTTGGAGTTGCTGAAGCTGAAAGGCAAGGCGCGAAAGCGTGCAAGAACCAAACAGAAGCCAGCGGAAGAAAA
GCCCTGGACGTGGGGTGACGAGTCGTGA

Structures

Toxin

• AlphaFold DB: B3EE80