Detailed information of TA system TA228971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 701777..702423
• Replicon: chromosome
• Accession: NZ_CP089777
• Organism: Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LVJ72_RS03155
• Protein ID: WP_031705732.1
• Coordinates: 701777..702190 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: LVJ72_RS03160
• Protein ID: WP_003403139.1
• Coordinates: 702187..702423 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ72_RS03135 (LVJ72_03130)	697860..699410	+	1551	WP_003403119.1	MlaD family protein	-
LVJ72_RS03140 (LVJ72_03135)	699462..699854	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
LVJ72_RS03145 (LVJ72_03140)	699851..700108	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
LVJ72_RS03150 (LVJ72_03145)	700291..701526	-	1236	WP_070898222.1	ATP-binding protein	-
LVJ72_RS03155 (LVJ72_03150)	701777..702190	-	414	WP_031705732.1	PIN domain-containing protein	Toxin
LVJ72_RS03160 (LVJ72_03155)	702187..702423	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
LVJ72_RS03165 (LVJ72_03160)	702527..703033	-	507	WP_003900973.1	two component system sensor kinase HK1	-
LVJ72_RS03170 (LVJ72_03165)	703147..703677	-	531	WP_003917320.1	two component system sensor kinase HK2	-
LVJ72_RS03175 (LVJ72_03170)	703661..704356	-	696	WP_003918099.1	two-component system response regulator TcrA	-
LVJ72_RS03180 (LVJ72_03175)	704479..704790	+	312	WP_003403164.1	hypothetical protein	-
LVJ72_RS03185 (LVJ72_03180)	704862..705812	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
LVJ72_RS03190 (LVJ72_03185)	706053..706637	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
LVJ72_RS03195 (LVJ72_03190)	706639..707349	+	711	Protein_633	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14756.12 Da
• Isoelectric Point: 8.1405

>T228971 WP_031705732.1 NZ_CP089777:c702190-701777 [Mycobacterium tuberculosis]
VKPPLAVDASVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T228971 NZ_CP124359:2963290-2963568 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT228971 WP_003403139.1 NZ_CP089777:c702423-702187 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT228971 NZ_CP124359:2963097-2963288 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA

Structures

Antitoxin

• AlphaFold DB: G0TQ89