Detailed information of TA system TA228953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3101000..3101570
• Replicon: chromosome
• Accession: NZ_CP089776
• Organism: Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: LVJ71_RS14780
• Protein ID: WP_003414166.1
• Coordinates: 3101000..3101356 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: LVJ71_RS14785
• Protein ID: WP_003901465.1
• Coordinates: 3101340..3101570 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ71_RS14760 (LVJ71_14765)	3096452..3098140	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
LVJ71_RS14765 (LVJ71_14770)	3098144..3098470	-	327	WP_003414157.1	hypothetical protein	-
LVJ71_RS14770 (LVJ71_14775)	3098643..3099230	+	588	WP_003914429.1	DUF3558 family protein	-
LVJ71_RS14775 (LVJ71_14780)	3099249..3100898	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
LVJ71_RS14780 (LVJ71_14785)	3101000..3101356	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
LVJ71_RS14785 (LVJ71_14790)	3101340..3101570	-	231	WP_003901465.1	type II toxin-antitoxin system antitoxin MazE9	Antitoxin
LVJ71_RS14790 (LVJ71_14795)	3101613..3102656	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
LVJ71_RS14795 (LVJ71_14800)	3102655..3103122	+	468	WP_003414177.1	DUF1778 domain-containing protein	-
LVJ71_RS14800 (LVJ71_14805)	3103151..3103405	-	255	WP_003917684.1	hypothetical protein	-
LVJ71_RS14805 (LVJ71_14810)	3103553..3103957	+	405	WP_009938577.1	hypothetical protein	-
LVJ71_RS14810 (LVJ71_14815)	3103954..3104145	+	192	WP_003414184.1	hypothetical protein	-
LVJ71_RS14815 (LVJ71_14820)	3104362..3104622	+	261	Protein_2924	transposase	-
LVJ71_RS14820 (LVJ71_14825)	3105732..3105989	+	258	WP_003899489.1	hypothetical protein	-
LVJ71_RS14825 (LVJ71_14830)	3106094..3106405	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T228953 WP_003414166.1 NZ_CP089776:c3101356-3101000 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T228953 NZ_CP124357:c4149883-4149596 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT228953 WP_003901465.1 NZ_CP089776:c3101570-3101340 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT228953 NZ_CP124357:c4149609-4149307 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3