Detailed information of TA system TA228924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1247339..1247907
• Replicon: chromosome
• Accession: NZ_CP089776
• Organism: Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: LVJ71_RS05980
• Protein ID: WP_003405865.1
• Coordinates: 1247533..1247907 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: LVJ71_RS05975
• Protein ID: WP_003405863.1
• Coordinates: 1247339..1247536 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ71_RS05955 (LVJ71_05950)	1243380..1244018	-	639	WP_003898730.1	lipid droplet-associated protein	-
LVJ71_RS05960 (LVJ71_05955)	1244108..1245115	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ71_RS05965 (LVJ71_05960)	1245132..1246115	-	984	WP_003898731.1	hypothetical protein	-
LVJ71_RS05970 (LVJ71_05965)	1246178..1247251	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
LVJ71_RS05975 (LVJ71_05970)	1247339..1247536	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ71_RS05980 (LVJ71_05975)	1247533..1247907	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ71_RS05985 (LVJ71_05980)	1248110..1248808	+	699	WP_003898733.1	hypothetical protein	-
LVJ71_RS05990 (LVJ71_05985)	1248926..1249111	+	186	WP_003901093.1	hypothetical protein	-
LVJ71_RS05995 (LVJ71_05990)	1249038..1249313	-	276	WP_003405867.1	hypothetical protein	-
LVJ71_RS06000 (LVJ71_05995)	1249556..1249879	+	324	WP_003405871.1	putative quinol monooxygenase	-
LVJ71_RS06005 (LVJ71_06000)	1249894..1250592	-	699	WP_031646953.1	hypothetical protein	-
LVJ71_RS06010 (LVJ71_06005)	1250626..1250835	+	210	WP_003911400.1	hypothetical protein	-
LVJ71_RS06015 (LVJ71_06010)	1250787..1251557	-	771	Protein_1185	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T228924 WP_003405865.1 NZ_CP089776:1247533-1247907 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T228924 NZ_CP124355:3589900-3590250 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT228924 WP_003405863.1 NZ_CP089776:1247339-1247536 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT228924 NZ_CP124355:3589655-3589906 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07