Detailed information of TA system TA228744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PhRel-ATphRel/-
• Location: 1547274..1548324
• Replicon: chromosome
• Accession: NZ_CP089773
• Organism: Mycobacterium tuberculosis strain 01-R1430

Toxin (Protein)

• Gene name: phRel
• Uniprot ID: -
• Locus tag: LVJ68_RS07325
• Protein ID: WP_003912801.1
• Coordinates: 1547274..1548095 (+)
• Length: 274 a.a.

Antitoxin (Protein)

• Gene name: ATphRel
• Uniprot ID: A0A7U4BTV8
• Locus tag: LVJ68_RS07330
• Protein ID: WP_003407167.1
• Coordinates: 1548088..1548324 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ68_RS07305 (LVJ68_07305)	1542832..1543494	-	663	WP_003407152.1	membrane protein	-
LVJ68_RS07310 (LVJ68_07310)	1543491..1544276	-	786	WP_003407157.1	membrane protein	-
LVJ68_RS07315 (LVJ68_07315)	1544567..1546528	-	1962	WP_003407159.1	sigma-F factor regulator	-
LVJ68_RS07320 (LVJ68_07320)	1546667..1547053	-	387	WP_003898845.1	anti-sigma-F factor antagonist RsfA	-
LVJ68_RS07325 (LVJ68_07325)	1547274..1548095	+	822	WP_003912801.1	GTP pyrophosphokinase family protein	Toxin
LVJ68_RS07330 (LVJ68_07330)	1548088..1548324	+	237	WP_003407167.1	hypothetical protein	Antitoxin
LVJ68_RS07335 (LVJ68_07335)	1548396..1549601	-	1206	WP_003898846.1	serine hydrolase domain-containing protein	-
LVJ68_RS07340 (LVJ68_07340)	1549904..1550689	+	786	WP_003407180.1	lipoprotein LprF	-
LVJ68_RS07345 (LVJ68_07345)	1550885..1552354	+	1470	WP_003900326.1	cytochrome b5 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (76-174)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 274 a.a.
• Molecular weight: 30134.12 Da
• Isoelectric Point: 5.8187

>T228744 WP_003912801.1 NZ_CP089773:1547274-1548095 [Mycobacterium tuberculosis]
VVVALVGSAIVDLHSRPPWSNNAVRRLGVALRDGVDPPVDCPSYAEVMLWHADLAAEVQDRIEGRSWSASELLVTSRAKS
QDTLLAKLRRRPYLQLNTIQDIAGVRIDADFLLGEQTRLAREIADHFGADQPAIHDLRDHPHAGYRAVHVWLRLPAGRVE
IQIRTILQSLWANFYELLADAYGRGIRYDERPEQLAAGVVPAQLQELVGVMQDASADLAMHEAEWQHCAEIEYPGQRAMA
LGEASKNKATVLATTKFRLERAINEAESAGGGG

Nucleotide

• Length: 822 bp

>T228744 NZ_CP124341:c825580-825203 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAAAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8830.90 Da
• Isoelectric Point: 7.3178

>AT228744 WP_003407167.1 NZ_CP089773:1548088-1548324 [Mycobacterium tuberculosis]
VGEVAGYVVEYNRRTHVRRITEFATPQEAMEHRLKLEAERTDSNIEIVALVSKSLGTLKQTHSRYFTGEELNVGNGAR

Nucleotide

• Length: 237 bp

>AT228744 NZ_CP124341:c826037-825657 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAACAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Antitoxin

• AlphaFold DB: A0A7U4BTV8